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NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 2)

Catalog # Concentration Size List Price Quantity Your Price
E7780S 96 reactions $726.00
$653.40
Catalog # Size List Price Your Price
E7780S 96 reactions $726.00
$653.40
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification. This kit includes 8 i5 index primers and 12 i7 index primers for dual indexing.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

  • High efficiency adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing
  • Increased sample identification specificity
 

Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER® Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing. This kit includes 8 i5 index primers and 12 i7 index primers.

NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.

Additional dual index primer options are available (NEB #E7600). Single primer options are also available, in 12- and 96- index formats (NEB #E7335, NEB #E7500, NEB #E7710, NEB #E7730, NEB #E6609). A methylated version of the NEBNext Adaptor is also available for use with bisulfite sequencing protocols (NEB #E7535). 


Dual Index Workflow
Dual Index Workflow

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  NEBNext Adaptor for Illumina E7601AVIAL -20 1 x 0.96 ml
  USER® Enzyme E7602AVIAL -20 1 x 0.288 ml
  NEBNext® i509 Primer E7781AVIAL -20 1 x 0.06 ml 10 µM
  NEBNext® i510 Primer E7782AVIAL -20 1 x 0.06 ml 10 µM
  NEBNext® i511 Primer E7783AVIAL -20 1 x 0.06 ml 10 µM
  NEBNext® i512 Primer E7784AVIAL -20 1 x 0.06 ml 10 µM
  NEBNext® i513 Primer E7785AVIAL -20 1 x 0.06 ml 10 µM
  NEBNext® i514 Primer E7786AVIAL -20 1 x 0.06 ml 10 µM
  NEBNext® i515 Primer E7787AVIAL -20 1 x 0.06 ml 10 µM
  NEBNext® i516 Primer E7788AVIAL -20 1 x 0.06 ml 10 µM
  NEBNext® i713 Primer E7789AVIAL -20 1 x 0.04 ml 10 µM
  NEBNext® i714 Primer E7790AVIAL -20 1 x 0.04 ml 10 µM
  NEBNext® i715 Primer E7791AVIAL -20 1 x 0.04 ml 10 µM
  NEBNext® i716 Primer E7792AVIAL -20 1 x 0.04 ml 10 µM
  NEBNext® i717 Primer E7793AVIAL -20 1 x 0.04 ml 10 µM
  NEBNext® i718 Primer E7794AVIAL -20 1 x 0.04 ml 10 µM
  NEBNext® i719 Primer E7795AVIAL -20 1 x 0.04 ml 10 µM
  NEBNext® i720 Primer E7796AVIAL -20 1 x 0.04 ml 10 µM
  NEBNext® i721 Primer E7797AVIAL -20 1 x 0.04 ml 10 µM
  NEBNext® i722 Primer E7798AVIAL -20 1 x 0.04 ml 10 µM
  NEBNext® i723 Primer E7799AVIAL -20 1 x 0.04 ml 10 µM
  NEBNext® i724 Primer E7800AVIAL -20 1 x 0.04 ml 10 µM
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

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The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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