Even more from less, for DNA
Are you challenged with trying to obtain higher library yields using ever-decreasing input amounts? Each component in the NEBNext® Ultra™ II DNA Library Prep Kit from NEB has been reformulated, resulting in a several-fold increase in library yield with as little as 500 picograms of input DNA. These advances deliver unprecedented performance, while enabling lower inputs and fewer PCR cycles.
Note: If your input DNA has not already been sheared, we recommend the NEBNext Ultra II FS DNA Library Prep Kit for Illumina, which fragments, end repairs, and dA-tails DNA, all in a single tube with no cleanups or transfers. This kit is available with and without sample purification beads.
The Ultra II DNA Library Prep Kit for Illumina meets the challenge of constructing high quality libraries from ever-decreasing input quantities. The reagents for each step in the library preparation workflow have been reformulated to enable high yield preparation of high quality libraries from 500 picograms to 1 microgram of input DNA. This new generation of NEBNext reagents uses a fast, streamlined, automatable workflow and enables use of fewer PCR cycles while also improving GC coverage. The kit is also compatible with PCR-free workflows and is effective with challenging samples such as FFPE DNA.
Now also available with optional SPRIselect® beads for clean-up and size-selection steps.To learn more about how NEBNext Ultra II addresses lower input amounts and challenging sample types, download our technical note.
For use with NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1) (NEB #E7395), refer to the Protocols tab for UMI Adaptors-specific guidance.
NEBNext Ultra II libraries provide the highest quality sequence data in PCR-free workflows
LIBRARY KIT | TOTAL READS | % DUPLICATION | % MAPPED | % CHIMERAS |
---|---|---|---|---|
Ultra II | 3,685,029 | 0.02 | 97.00 | 1.10 |
Kapa Hyper | 3,679,136 | 0.02 | 97.00 | 2.00 |
TruSeq PCR-Free | 3,681,643 | 0.05 | 96.65 | 1.38 |
FFPE DNA | DNA INPUT (ng) | LIBRARY YIELDS IN ng | % MAPPED | % MAPPED IN PAIRS | % DUPLICATION | % CHIMERAS |
---|---|---|---|---|---|---|
Kidney Tumor | 17 | 132 | 91.5 | 96.1 | 0.48 | 3.0 |
Lung Tumor | 20 | 232 | 90.1 | 94.9 | 0.42 | 4.1 |
Liver Normal | 20 | 691 | 92.6 | 94.7 | 0.33 | 8.6 |
Breast Tumor | 30 | 514 | 91.9 | 95.1 | 0.37 | 4.5 |
View additional data on target enrichment
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
E7645S | -20 |
NEBNext® Ultra II End Prep Enzyme Mix | E7646AVIAL | -20 | 1 x 0.072 ml | ||
NEBNext® Ultra II End Prep Reaction Buffer | E7647AVIAL | -20 | 1 x 0.168 ml | ||
NEBNext® Ultra™ II Ligation Master Mix | E7648AVIAL | -20 | 1 x 0.72 ml | ||
NEBNext® Ligation Enhancer | E7374AVIAL | -20 | 1 x 0.024 ml | ||
NEBNext® Ultra II Q5® Master Mix | E7649AVIAL | -20 | 1 x 0.6 ml |
E7645L | -20 |
NEBNext® Ultra II End Prep Enzyme Mix | E7646AAVIAL | -20 | 1 x 0.288 ml | ||
NEBNext® Ultra II End Prep Reaction Buffer | E7647AAVIAL | -20 | 1 x 0.672 ml | ||
NEBNext® Ultra™ II Ligation Master Mix | E7648AAVIAL | -20 | 3 x 0.96 ml | ||
NEBNext® Ligation Enhancer | E7374AAVIAL | -20 | 1 x 0.096 ml | ||
NEBNext® Ultra II Q5® Master Mix | E7649AAVIAL | -20 | 2 x 1.2 ml |
For NEB #E7645 only:
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
This product is covered by one or more patents.
This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows.
For additional information or to inquire about commercial use, please contact busdev@neb.com.