This module is part of the Ultra™ II workflow, and is optimized for use with the NEBNext® Ultra II End Repair/dA-Tailing Module (NEB #E7546), for Illumina®-compatible library construction.
The NEBNext Ultra II Ligation Module is optimized for use with the NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546) or the NEBNext Ultra II FS DNA Module (NEB #E7810).
This module is also compatible with some Oxford Nanopore Technologies® workflows, including some involving COVID-19 research.
The NEBNext Ultra II Ligation Module has been optimized to ligate efficiently DNA adaptors compatible with Illumina sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546), and is part of the Ultra II DNA workflow which enables high yield preparation of high quality libraries from 500 pg to 1 µg of input DNA.
This module is compatible with Illumina workflows and with several Oxford Nanopore Technologies workflows.
For Illumina library construction, the NEBNext Ultra II Ligation Module is designed for use with the following:
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.