NEBNext® Ultra™ II DNA Library Prep with Sample Purification Beads

Catalog #	Concentration	Size	List Price	Quantity	Your Price
E7103L		96 reactions	$3,754.00		$3,378.60
E7103S		24 reactions	$983.00		$884.70

Catalog #	Size	List Price	Your Price
E7103L	96 reactions	$3,754.00	$3,378.60
E7103S	24 reactions	$983.00	$884.70

Even more from less, for DNA

Are you challenged with trying to obtain higher library yields using ever-decreasing input amounts? Each component in the NEBNext Ultra II DNA Library Prep Kit from NEB has been reformulated, resulting in a several-fold increase in library yield with as little as 500 picograms of input DNA. These advances deliver unprecedented performance, while enabling lower inputs and fewer PCR cycles.

Get more of what you need, with the highest library yields
Use to generate high quality libraries from a broad range of input amounts, from 500 pg to 1 µg
Prepare libraries from ALL your samples, including GC-rich DNA and FFPE DNA samples
Improve yields and quality for target enrichment applications
Save time with streamlined workflows, reduced hands-on time and automation compatibility, and enjoy the flexibility of kit or module formats
Enjoy the flexibility and reliability of the gold standard SPRIselect^® size selection and clean-up beads, supplied in just the amounts you need

Note: If your input DNA has not already been sheared, we recommend the NEBNext Ultra II FS DNA Library Prep Kit for Illumina, which fragments, end repairs, and dA-tails DNA, all in a single tube with no cleanups or transfers. This kit is available with and without sample purification beads..

The NEBNext Ultra II DNA Library Prep with Sample Purification Beads contains the enzymes, buffers and purification beads required to convert a broad range of DNA input amounts into indexed libraries for next-generation sequencing on the Illumina platform. The fast, user-friendly workflow has minimal hands-on time and, for added convenience, this kit contains NEBNext Sample Purification Beads (SPRIselect^® beads from Beckman Coulter) for size selection and enzyme reaction cleanup.

Advantages

Get more of what you need, with the highest library yields
Use to generate high quality libraries even when you have only limited amounts of DNA, with inputs as low as 500 pg
Prepare libraries from ALL of your samples, including GC-rich targets and FFPE DNA samples
Improve yields and quality for target enrichment applications
Save time with streamlined workflows, reduced hands-on time and automation compatibility, and enjoy the flexibility of kit or module formats
Enjoy the flexibility and reliability of the gold standard SPRIselect size selection and clean-up beads, supplied in just the amounts you need

To learn more about how NEBNext Ultra II addresses lower input amounts and challenging sample types, download our technical note.

For use with NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1) (NEB #E7395), refer to the Protocols tab for UMI Adaptors-specific guidance.

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

E7103S Multi-temperature

	NEBNext^® Ultra™ II DNA Library Prep Kit for Illumina^®	E7645S	-20	1 x 24 reactions
	NEBNext^® Sample Purification Beads	E7104S	25	1 x 4 ml

E7103L Multi-temperature

	NEBNext^® Ultra™ II DNA Library Prep Kit for Illumina^®	E7645L	-20	1 x 96 reactions
	NEBNext^® Sample Purification Beads	E7104L	25	1 x 16 ml

Properties & Usage

Materials Required but not Supplied

80% Ethanol (freshly prepared)
Nuclease-free Water
0.1X TE (1 mM Tris-HCl, pH 8.0, 0.1 mM EDTA)
DNA LoBind Tubes (Eppendorf #022431021)
NEBNext Multiplex Oligos for Illumina (for a full list, visit www.neb.com/oligos)
Magnetic rack or plate (e.g., NEBNext^® Magnetic Separation Rack (NEB #S1515S), Alpaqua ^® 96S Super Magnet Plate (#A001322), or equivalent)
PCR Machine

Optional:

10 mM Tris-HCl, pH 8.0 or
Tris/NaCl, pH 8.0 (10 mM Tris, 10 mM NaCl)
(for adaptor dilution of DNA input ≤ 100 ng)

Storage Temperature:
Package 1: NEBNext Ultra II DNA Library Prep Kit for Illumina - Store at -20°C
Package 2: NEBNext Sample Purification Beads - Store at Room Temperature. Do not freeze.

Protocols

Where can I find protocols for using NEBNext® DNA Library Prep reagents?

DataCards

FAQs

Tech Tips

When removing the supernatant from the magnetic beads during cleanup, it is important not to disturb the pellet. Find these and other helpful tips for optimizing the size selection and clean up steps in this protocol video.

Citations

See more details on Bioz

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Safety Data Sheets

Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows.

For additional information or to inquire about commercial use, please contact busdev@neb.com.