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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

Catalog # Concentration Size List Price Quantity Your Price
E7500L 96 reactions $608.00
$547.20
E7500S 24 reactions $165.00
$148.50
Catalog # Size List Price Your Price
E7500L 96 reactions $608.00
$547.20
E7500S 24 reactions $165.00
$148.50
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

NEBNext® Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina® library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

  • High efficiency adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing

Note: NEBNext® Multiplex Oligos for Illumina® (Index Primer Set 1) (NEB #7335S) includes 12 indices. 

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Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER™ Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing.

NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.

Additional single- or dual-barcode primer options are available, in 12- and 96- index formats (NEB #E7335, NEB #E7710, NEB #E7730, NEB #E6609, NEB #E7600). A methylated version of the NEBNext Adaptor is also available for use with bisulfite sequencing protocols (NEB #E7535).


Figure 1: Workflow
Figure 1: Workflow


Functional Validation

Each set of reagents is functionally validated together through construction and sequencing of genomic DNA libraries on the Illumina sequencing platform.

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  NEBNext Adaptor for Illumina E7337AVIAL -20 1 x 0.24 ml
  USER® Enzyme E7338AVIAL -20 1 x 0.072 ml
  NEBNext Universal PCR Primer for Illumina E6861AVIAL -20 1 x 0.12 ml 10 µM
  NEBNext Index 13 Primer for Illumina E6862AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 14 Primer for Illumina E6863AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 15 Primer for Illumina E6864AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 18 Primer for Illumina E6866AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 19 Primer for Illumina E6867AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 20 Primer for Illumina E6868AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 21 Primer for Illumina E6869AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 22 Primer for Illumina E6870AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 23 Primer for Illumina E6871AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 25 Primer for Illumina E6872AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 27 Primer for Illumina E6873AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 16 Primer for Illumina E7511AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Adaptor for Illumina E7337AAVIAL -20 1 x 0.96 ml
  USER® Enzyme E7338AAVIAL -20 1 x 0.288 ml
  NEBNext Universal PCR Primer for Illumina E6861AAVIAL -20 1 x 0.48 ml 10 µM
  NEBNext Index 13 Primer for Illumina E6862AAVIAL -20 1 x 0.04 ml 10 µM
  NEBNext Index 14 Primer for Illumina E6863AAVIAL -20 1 x 0.04 ml 10 µM
  NEBNext Index 15 Primer for Illumina E6864AAVIAL -20 1 x 0.04 ml 10 µM
  NEBNext Index 18 Primer for Illumina E6866AAVIAL -20 1 x 0.04 ml 10 µM
  NEBNext Index 19 Primer for Illumina E6867AAVIAL -20 1 x 0.04 ml 10 µM
  NEBNext Index 20 Primer for Illumina E6868AAVIAL -20 1 x 0.04 ml 10 µM
  NEBNext Index 21 Primer for Illumina E6869AAVIAL -20 1 x 0.04 ml 10 µM
  NEBNext Index 22 Primer for Illumina E6870AAVIAL -20 1 x 0.04 ml 10 µM
  NEBNext Index 23 Primer for Illumina E6871AAVIAL -20 1 x 0.04 ml 10 µM
  NEBNext Index 25 Primer for Illumina E6872AAVIAL -20 1 x 0.04 ml 10 µM
  NEBNext Index 27 Primer for Illumina E6873AAVIAL -20 1 x 0.04 ml 10 µM
  NEBNext Index 16 Primer for Illumina E7511AAVIAL -20 1 x 0.04 ml 10 µM

Properties & Usage

Materials Required but not Supplied

For the list of additional materials required, please check the manual for your NEBNext Library Prep Kit.

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Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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