NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

Catalog #	Concentration	Size	List Price	Quantity	Your Price
E7710L		96 reactions	$608.00		$547.20
E7710S		24 reactions	$165.00		$148.50

Catalog #	Size	List Price	Your Price
E7710L	96 reactions	$608.00	$547.20
E7710S	24 reactions	$165.00	$148.50

Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER^®Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing.

NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.

Additional single- or dual-barcode primer options are available, in 12- and 96- index formats (NEB #E7335, NEB #E7500, NEB #E7730, NEB #E6609, NEB #E7600). A methylated version of the NEBNext Adaptor is also available for use with bisulfite sequencing protocols (NEB #E7535).

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

E7710S -20

NEBNext Index 26 Primer for Illumina	E7713AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 28 Primer for Illumina	E7714AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 29 Primer for Illumina	E7715AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 31 Primer for Illumina	E7717AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 32 Primer for Illumina	E7718AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 33 Primer for Illumina	E7719AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 34 Primer for Illumina	E7720AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 35 Primer for Illumina	E7721AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 36 Primer for Illumina	E7722AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Universal PCR Primer for Illumina	E6861AVIAL	-20	1 x 0.12 ml	10 µM
NEBNext Adaptor for Illumina	E7337AVIAL	-20	1 x 0.24 ml
USER^® Enzyme	E7338AVIAL	-20	1 x 0.072 ml
NEBNext Index 42 Primer for Illumina	E7736AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 24 Primer for Illumina	E7592AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 17 Primer for Illumina	E7585AVIAL	-20	1 x 0.01 ml	10 µM

E7710L -20

NEBNext Adaptor for Illumina	E7337AAVIAL	-20	1 x 0.96 ml
USER^® Enzyme	E7338AAVIAL	-20	1 x 0.288 ml
NEBNext Universal PCR Primer for Illumina	E6861AAVIAL	-20	1 x 0.48 ml	10 µM
NEBNext Index 17 Primer for Illumina	E7585AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 24 Primer for Illumina	E7592AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 26 Primer for Illumina	E7713AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 28 Primer for Illumina	E7714AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 29 Primer for Illumina	E7715AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 31 Primer for Illumina	E7717AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 32 Primer for Illumina	E7718AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 33 Primer for Illumina	E7719AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 34 Primer for Illumina	E7720AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 35 Primer for Illumina	E7721AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 36 Primer for Illumina	E7722AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 42 Primer for Illumina	E7736AAVIAL	-20	1 x 0.04 ml	10 µM

Properties & Usage

Materials Required but not Supplied

For the list of additional materials required, please check the manual for your NEBNext Library Prep Kit.

Companion Products

Usage Guidelines

DataCards

manualE7335_E7500_ E7710_E7730

Selection Charts

NEBNext® Multiplex Oligos Selection Chart

FAQs

Citations

See more details on Bioz

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

E7710S_L_v1

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
This product is covered by one or more patents.

This product is licensed from Bio-Rad Laboratories, Inc. under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, 7,919,296, 8,232,078, 8,367,376, 8,415,129, 8,445,249, 8,470,573, 8,476,045, 8,895,283 and 8,900,846 and corresponding patents in other countries for use only in: (a) standard (non-real time) PCR in the research field only, but not digital PCR; (b) real-time PCR for internal product research and development purposes only, and not for sales to end-users within the research field; (c) any in vitro diagnostics application, including applications using real-time PCR but not digital PCR; and (d) any non-PCR applications in DNA sequencing, isothermal amplification and the production of synthetic DNA.