NEBNext® Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification.
For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.
Note: NEBNext® Multiplex Oligos for Illumina® (Index Primer Set 1) ( NEB #7335) includes 12 indices.
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
E7730S | -20 |
USER® Enzyme | E7338AVIAL | -20 | 1 x 0.072 ml | ||
NEBNext Adaptor for Illumina | E7337AVIAL | -20 | 1 x 0.24 ml | ||
NEBNext Universal PCR Primer for Illumina | E6861AVIAL | -20 | 1 x 0.12 ml | 10 µM | |
NEBNext Index 37 Primer for Illumina | E7731AVIAL | -20 | 1 x 0.01 ml | 10 µM | |
NEBNext Index 38 Primer for Illumina | E7732AVIAL | -20 | 1 x 0.01 ml | 10 µM | |
NEBNext Index 39 Primer for Illumina | E7733AVIAL | -20 | 1 x 0.01 ml | 10 µM | |
NEBNext Index 41 Primer for Illumina | E7735AVIAL | -20 | 1 x 0.01 ml | 10 µM | |
NEBNext Index 43 Primer for Illumina | E7737AVIAL | -20 | 1 x 0.01 ml | 10 µM | |
NEBNext Index 44 Primer for Illumina | E7738AVIAL | -20 | 1 x 0.01 ml | 10 µM | |
NEBNext Index 45 Primer for Illumina | E7739AVIAL | -20 | 1 x 0.01 ml | 10 µM | |
NEBNext Index 46 Primer for Illumina | E7740AVIAL | -20 | 1 x 0.01 ml | 10 µM | |
NEBNext Index 47 Primer for Illumina | E7741AVIAL | -20 | 1 x 0.01 ml | 10 µM | |
NEBNext Index 48 Primer for Illumina | E7742AVIAL | -20 | 1 x 0.01 ml | 10 µM | |
NEBNext® Index 40 Primer for Illumina® | E7744AVIAL | -20 | 1 x 0.01 ml | 10 µM | |
NEBNext Index 30 Primer for Illumina | E7716AVIAL | -20 | 1 x 0.01 ml | 10 µM |
E7730L | -20 |
USER® Enzyme | E7338AAVIAL | -20 | 1 x 0.288 ml | ||
NEBNext Adaptor for Illumina | E7337AAVIAL | -20 | 1 x 0.96 ml | ||
NEBNext Universal PCR Primer for Illumina | E6861AAVIAL | -20 | 1 x 0.48 ml | 10 µM | |
NEBNext Index 30 Primer for Illumina | E7716AAVIAL | -20 | 1 x 0.04 ml | 10 µM | |
NEBNext Index 37 Primer for Illumina | E7731AAVIAL | -20 | 1 x 0.04 ml | 10 µM | |
NEBNext Index 38 Primer for Illumina | E7732AAVIAL | -20 | 1 x 0.04 ml | 10 µM | |
NEBNext Index 39 Primer for Illumina | E7733AAVIAL | -20 | 1 x 0.04 ml | 10 µM | |
NEBNext® Index 40 Primer for Illumina® | E7744AAVIAL | -20 | 1 x 0.04 ml | 10 µM | |
NEBNext Index 41 Primer for Illumina | E7735AAVIAL | -20 | 1 x 0.04 ml | 10 µM | |
NEBNext Index 43 Primer for Illumina | E7737AAVIAL | -20 | 1 x 0.04 ml | 10 µM | |
NEBNext Index 44 Primer for Illumina | E7738AAVIAL | -20 | 1 x 0.04 ml | 10 µM | |
NEBNext Index 45 Primer for Illumina | E7739AAVIAL | -20 | 1 x 0.04 ml | 10 µM | |
NEBNext Index 46 Primer for Illumina | E7740AAVIAL | -20 | 1 x 0.04 ml | 10 µM | |
NEBNext Index 47 Primer for Illumina | E7741AAVIAL | -20 | 1 x 0.04 ml | 10 µM | |
NEBNext Index 48 Primer for Illumina | E7742AAVIAL | -20 | 1 x 0.04 ml | 10 µM |
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