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NEB New Products

Release Date:12/12/2024
NEBNext® Enzymatic Methyl-seq v2 Conversion Module

The NEBNext® Enzymatic Methyl-seq v2 Conversion Module contains reagents for enzyme-based conversion for methylome analysis, with an expanded input range.

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Release Date:12/03/2024
RNase 4 Digestion and 3´ End Repair Mix

RNase 4 Digestion and 3´ End Repair Mix is a coformulation of RNase 4 and T4 Polynucleotide Kinase that simplifies RNase 4 digestion products into a pool of U-ending RNA oligonucleotides with homogeneous 3´-hydroxy termini.

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Release Date:11/04/2024
Thermostable Endonuclease Q

Thermostable Endonuclease Q is an archaeal DNA endonuclease that cleaves the phosphate backbone of a DNA substrate 5′ to the position of the modified nucleobases of deoxyxanthosine (dX), deoxyinosine (dI), deoxyuracil (dU), and an abasic (AP) site. The result is a 3′-OH and a 5′-phosphate.

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Release Date:10/01/2024
NEBExpress® Salt Active Nuclease

Proprietary endonuclease that non-specifically cleaves and degrades all forms of DNA and RNA, with optimal activity in high salt (500 mM NaCl/KCl).

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Release Date:09/10/2024
N1-Methyl-Pseudouridine-5’-Triphosphate (N1-Methyl-Pseudo-UTP)

mRNA has emerged as a potent modality for therapeutics. Incorporation of chemically modified ribonucleotides improves the efficacy of these therapies by increasing stability, reducing immunogenicity, and enhancing translatability of mRNAs.

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Release Date:09/10/2024
5-Methoxy-Uridine-5’-Triphosphate (5-Methoxy-UTP)

Modified NTPs are commonly used for reduction of immunogenicity for in vitro transcription RNA.

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Release Date:09/10/2024
5-Methyl-Cytidine-5’-Triphosphate (5-Methyl-CTP)

Modified NTPs are commonly used for reduction of immunogenicity for in vitro transcription RNA.

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Release Date:09/10/2024
Pseudouridine-5’-Triphosphate (Pseudo-UTP)

Modified NTPs are commonly used for reduction of immunogenicity for in vitro transcription RNA.

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Release Date:08/19/2024
NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing

This new module includes the reagents recommended in the updated Oxford Nanopore Technologies® SQK-LSK114 singleplex ligation sequencing library prep protocols.

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Release Date:08/01/2024
phi29-XT WGA Kit

The phi29-XT WGA Kit contains the necessary components to perform whole genome amplification (WGA), including phi29-XT DNA Polymerase, an engineered polymerase with improved thermostability and sensitivity.

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Release Date:07/23/2024
Monarch® Spin PCR & DNA Cleanup Kit (5 μg)

New version of PCR cleanup kit, featuring upgraded spin columns, for rapid and reliable purification and concentration of DNA up to 5 from various sample types.

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Release Date:07/23/2024
Monarch® Spin DNA Gel Extraction Kit

New version of gel extraction kit, featuring upgraded spin columns, provides a rapid and reliable method for extraction of DNA from agarose gels.

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Release Date:07/22/2024
NEBNext® RNA Library Prep Kit for MGI®

NEBNext® RNA library prep reagents are now available for MGI sequencing applications.Kit components and protocols have been optimized for the production of high-quality directional RNA libraries from a wide range of inputs (10 ng – 1 µg).

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Release Date:07/22/2024
NEBNext® FS DNA Library Prep Kit for MGI®

NEBNext® FS DNA library prep reagents are now available for MGI DNBSEQ® sequencing applications.Kit components and protocols have been optimized for the production of high-quality DNA libraries from a wide range of intact DNA inputs (0.1 ng – 500 ng).

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Release Date:07/22/2024
NEBNext® Multiplex Oligos for MGI® (Dual Index Primer Pairs Set 1)

The NEBNext Multiplex Oligos for MGI (Dual Index Primer Pairs Set 1) streamlines MGI library production through its pre-selected, dual-index primer pairs delivered in a convenient 96-well plate format. This product is available in two sizes: the small pack (NEB #E9725S) with 24 primer pairs and the large pack (NEB #E9725L) with 96 primer pairs, which can be combined to index up to 120 samples.

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Release Date:07/22/2024
NEBNext® Circularization Module for MGI®

The NEBNext Circularization Module for MGI is optimized for the circularization of double-stranded DNA MGI-compatible libraries to create single-stranded circular DNA libraries for sequencing on the MGI platform.

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Release Date:06/12/2024
Monarch® Spin Plasmid Miniprep Kit

The new version of Monarch® Spin Plasmid Miniprep Kit enables highly concentrated DNA in extraction and isolation of plasmid DNA from bacterial host strains.

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Release Date:06/12/2024
Monarch® Spin Columns S2D and Tubes

Monarch Spin S2D Columns and Collection Tubes for nucleic acid purification, plasmid purification.

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Release Date:04/24/2024
LyoPrime WarmStart™ Fluorescent LAMP/RT-LAMP Mix (with UDG)

The LyoPrime WarmStart® Fluorescent LAMP/RT-LAMP Mix (with UDG) is a simple, one-step solution for real-time fluorescent detection of LAMP/RT-LAMP reactions.

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Release Date:03/27/2024
Monarch® Mag Viral DNA/RNA Extraction Kit

The Monarch Mag® Viral DNA/RNA Extraction Kit provides a rapid and reliable magnetic bead-based process for extracting viral nucleic acids from saliva and respiratory swab samples.

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Release Date:03/27/2024
Monarch StabiLyse™ DNA/RNA Buffer

Monarch buffer for DNA and RNA stabilization and protection in samples. Dual-function as sample lysis solution.

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Release Date:03/18/2024
N-Glycopeptide Binding Protein

Selectively bind and enrich N-glycopeptides, without using HILIC columns, for N-glycoprotein identification and discovery.

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Release Date:03/11/2024
Cryogenic Cold Storage Box

The Cryogenic Cold Storage Box from NEB is a cryo box designed to store and protect vials and microcentrifuge tubes in -80°C and -20°C freezers, 4°C fridges, and at room temperature. The box measures a height of 2 inches and can hold 81 tubes.

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Release Date:03/07/2024
RNase 4

RNase 4 is a single-stranded RNA endonuclease that cleaves 3´ of uridine in uridine-purine sequences (cut sites: U/A and U/G).

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Release Date:03/05/2024
Bst 2.0 WarmStart® DNA Polymerase (Glycerol-free)

A homolog of Bst DNA Polymerase, Large Fragment with an aptamer that inhibits polymerase activity at temperatures < 45°C in a glycerol-free format.

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Release Date:03/05/2024
WarmStart® RTx Reverse Transcriptase (Glycerol-free)

In silico-designed RNA-directed DNA polymerase coupled with a reversibly-bound aptamer that inhibits RTx activity below 40°C in a glycerol-free format.

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Release Date:02/20/2024
EnGen® SpRY Cas9

EnGen® SpRY Cas9 from Streptococcus pyogenes is an engineered, RNA-guided, DNA endonuclease that catalyzes site-specific cleavage of double-stranded DNA (dsDNA).

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Release Date:02/12/2024
NEBNext® Primers for Epigenetics (Unique Dual Index Set 3)

96 unique index primer pairs optimized for NEBNext® E5hmC-seq™ workflows and Illumina® sequencing

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Release Date:02/12/2024
NEBNext® Primers for Epigenetics (Unique Dual Index Set 2B)

24 unique index primer pairs optimized for NEBNext® E5hmC-seq™ and Illumina® sequencing

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Release Date:02/12/2024
NEBNext® Enzymatic 5hmC-seq Kit

The NEBNext® Enzymatic 5hmC-seq Kit enables enzyme-based specific detection of 5hmC at the single base level with Illumina® sequencing, through construction of libraries that are enzymatically modified to specifically detect 5hmC.

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Release Date:02/12/2024
NEBNext® Enzymatic 5hmC-seq Conversion Module

The NEBNext® Enzymatic 5hmC-seq Conversion Module contains reagents for enzyme-based specific detection of 5hmC.

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Release Date:02/01/2024
Duplex DNase

Duplex DNase is an engineered double-strand specific DNA endonuclease that preferentially degrades double-stranded DNA (dsDNA) over single-stranded DNA (ssDNA) or RNA. It will also cleave the DNA strand of DNA:RNA hybrid duplex.

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Release Date:12/21/2023
NEBNext UltraExpress® FS DNA Library Prep Kit

NEBNext UltraExpress is the next generation of NGS library prep, and the NEBNext UltraExpress FS DNA Library Prep Kit offers a one-size-fits-all protocol (within the stated input range) for simplified and streamlined library prep, including enzymatic fragmentation, from sample to high-quality library in under two hours!

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Release Date:11/27/2023
WarmStart® Afu Uracil-DNA Glycosylase (UDG)

WarmStart Afu Uracil-DNA Glycosylase (UDG) from Archaeoglobus fulgidus efficiently catalyzes the release of free uracil from uracil-containing single-stranded or double-stranded DNA at temperatures >42°C.

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Release Date:10/25/2023
NEBNext UltraExpress® DNA Library Prep Kit

NEBNext UltraExpress is the next generation of NGS library prep, and the NEBNext UltraExpress DNA Library Prep Kit offers a one-size-fits-all protocol (within the stated input range) for simplified and streamlined library prep, from sample to high-quality library in under two hours!

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Release Date:10/25/2023
NEBNext UltraExpress® RNA Library Prep Kit

The NEBNext UltraExpress RNA Library Prep Kit is the latest generation of NEBNext RNA library prep. With a streamlined 3-hour library prep protocol, the kit enables creation of high-quality RNA libraries in a single day, in conjunction with mRNA enrichment or rRNA depletion kits.

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Release Date:09/06/2023
Authenticase®

Authenticase is a mixture of structure-specific nucleases capable of recognizing and cleaving outside mismatch and indel regions from 1-10 bp on double-stranded DNA. The formulation has limited non-specific activity on homoduplex regions of DNA.

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Release Date:07/25/2023
NEBNext® FFPE DNA Library Prep Kit

This kit is designed for NGS library prep of pre-sheared FFPE DNA and includes reagents for repair and optimized library prep.

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Release Date:07/25/2023
NEBNext Ultrashear® FFPE DNA Library Prep Kit

This kit for FFPE DNA library prep combines specialized enzymatic FFPE DNA fragmentation and repair, with optimized reagents and protocols to provide superior performance with this challenging sample type.

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Release Date:06/28/2023
NEBNext UltraShear®

This new enzyme mix for DNA fragmentation has been designed for use with challenging samples, including FFPE DNA and methylated DNA. NEBNext UltraShear maintains methylation marks on DNA.

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Release Date:06/14/2023
EnGen® Seq1 Cas9

EnGen Seq1 Cas9 from Streptococcus equinus is an RNA-guided DNA endonuclease that catalyzes site-specific cleavage of double-stranded DNA (dsDNA).

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Release Date:05/11/2023
NEBNext® Multiplex Oligos for Illumina® (Unique Dual Index UMI Adaptors DNA Set 3)

The third set of Unique Dual Index UMI Adaptors for improved SNV detection or PCR-free workflows.

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Release Date:05/11/2023
NEBNext® Multiplex Oligos for Illumina® (Unique Dual Index UMI Adaptors DNA Set 4)

The fourth set of Unique Dual Index UMI Adaptors for improved SNV detectiPCRon or PCR-free workflows.

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Release Date:05/11/2023
NEBNext® Multiplex Oligos for Illumina® (Unique Dual Index UMI Adaptors DNA Set 2)

The second set of Unique Dual Index UMI Adaptors for improved SNV detection or PCR-free workflows.

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Release Date:05/03/2023
NEBExpress® T4 Lysozyme

NEBExpress® T4 Lysozyme is a recombinant murein hydrolase that breaks down the bacterial cell wall by hydrolyzing the -1,4 linkage between N-acetylmuramic acid and N-acetylglucosamine in the peptidoglycan of prokaryotic cells (gram-negative and some gram-positive bacteria).  It can be used to extract soluble proteins, membrane proteins, DNA, RNA, or metabolites.

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Release Date:04/04/2023
phi29-XT RCA Kit

This kit contains the components for optimized RCA: phi29-XT DNA Polymerase engineered for improved performance, exo-resistant random primers, and dNTPs.

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Release Date:03/23/2023
NEBNext® High Input Poly(A) mRNA Isolation Module

Isolate intact poly(A)+ RNA from high inputs (5-50 µg) of previously isolated total RNA, using oligo d(T)25-coupled paramagnetic beads. Intact mRNA can be obtained in approximately one hour, and eluted in small volumes. 

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Release Date:02/14/2023
Thermostable OGG

Thermostable OGG is a bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities.

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