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NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

Catalog # Concentration Size List Price Quantity Your Price
E6609L 384 reactions $4,211.00
$3,789.90
E6609S 96 reactions $1,053.00
$947.70
Catalog # Size List Price Your Price
E6609L 384 reactions $4,211.00
$3,789.90
E6609S 96 reactions $1,053.00
$947.70
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

NEBNext® Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina® library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification.

The 96 8-base index primers included in this kit are pre-mixed with the universal primer and are packaged in a single-use 96-well plate with a pierceable foil seal.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

  • High efficiency adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing
  • 96-well plate format with pierceable foil seal
Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER® Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing.

The 96 8-base index primers included in this kit are pre-mixed with the universal primer and are packaged in a single-use 96-well plate with a pierceable foil seal.

NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.

Additional single- or dual-barcode primer options are available, in 12- and 96- index formats (NEB #E7335, NEB #E7500, NEB #E7710, NEB #E7730, NEB #E7600). A methylated version of the NEBNext Adaptor is also available for use with bisulfite sequencing protocols (NEB #E7535).

Figure 1: Workflow demonstrating the use of NEBNext Multiplex Oligos for Illumina (96 Index Primers)

Figure 1: Workflow


Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  USER® Enzyme E6610AVIAL -20 1 x 0.288 ml
  NEBNext® Index/Universal Primer Mix (1-96) E6611AVIAL -20 1 x 10 μl/well 10 µM
  NEBNext® Adaptor for Illumina® E6612AVIAL -20 1 x 0.96 ml 15 µM
  USER® Enzyme E6610AAVIAL -20 2 x 0.576 ml
  NEBNext® Index/Universal Primer Mix (1-96) E6611AVIAL -20 4 x 10 μl/well 10 µM
  NEBNext® Adaptor for Illumina® E6612AVIAL -20 4 x 0.96 ml 15 µM
NEBNext® Multiplex Oligos for Illumina® (96 Index Primers) | New England Biolabs
Home Next Generation Sequencing Library Preparation Products NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

NEBNext® Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina® library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification.

The 96 8-base index primers included in this kit are pre-mixed with the universal primer and are packaged in a single-use 96-well plate with a pierceable foil seal.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

  • High efficiency adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing
  • 96-well plate format with pierceable foil seal
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Catalog https://www.neb.com/en/# Concentration Size
E6609S Not Applicable 96 reactions
E6609L Not Applicable 384 reactions
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New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.NEW ENGLAND BIOLABS® and NEBNEXT® are registered trademarks of New England Biolabs, Inc.
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