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The NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina provides a fragmentation system: a fast and reliable solution for DNA fragmentation and library construction. A new DNA fragmentation reagent is combined with end repair and dA-tailing reagents, allowing these steps to be performed in the same tube, with no clean-ups or sample loss. The same fragmentation protocol is used for any input amount (100 pg–500 ng), or GC content. The kit also includes the NEBNext Ultra II Ligation Master Mix for adaptor ligation, and the NEBNext Ultra II Q5® Master Mix for uniform, high-fidelity library amplification. Please note that adaptors and primers are not included in the kit and are available separately.
For protocols including bisulfite converted DNA, we recommend the NEBNext Ultra II DNA Library Prep Kit for Illumina.
Features:
Download extensive performance data in our technical note.
Also available with optional SPRIselect® beads for size selection/clean-up steps
For use with NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1) (NEB #E7395), refer to the Protocols tab for UMI Adaptors-specific guidance.
View additional data presented at AGBT by Peter Ellis, Senior Staff Scientist at the Wellcome Trust Sanger Institute.
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
E7805S | -20 |
NEBNext® Ligation Enhancer | E7374AVIAL | -20 | 1 x 0.024 ml | ||
NEBNext® Ultra™ II Ligation Master Mix | E7648AVIAL | -20 | 1 x 0.72 ml | ||
NEBNext® Ultra II Q5® Master Mix | E7649AVIAL | -20 | 1 x 0.6 ml | ||
NEBNext® Ultra™ II FS Enzyme Mix | E7806AVIAL | -20 | 1 x 0.048 ml | ||
NEBNext® Ultra™ II FS Reaction Buffer | E7807AVIAL | -20 | 1 x 0.168 ml | ||
TE Buffer (1X) | E7808AVIAL | -20 | 1 x 1.1 ml | 1 X |
E7805L | -20 |
NEBNext® Ligation Enhancer | E7374AAVIAL | -20 | 1 x 0.096 ml | ||
NEBNext® Ultra™ II Ligation Master Mix | E7648AAVIAL | -20 | 3 x 0.96 ml | ||
NEBNext® Ultra II Q5® Master Mix | E7649AAVIAL | -20 | 2 x 1.2 ml | ||
NEBNext® Ultra™ II FS Enzyme Mix | E7806AAVIAL | -20 | 1 x 0.192 ml | ||
NEBNext® Ultra™ II FS Reaction Buffer | E7807AAVIAL | -20 | 1 x 0.672 ml | ||
TE Buffer (1X) | E7808AAVIAL | -20 | 1 x 4.3 ml | 1 X |
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows.
For additional information or to inquire about commercial use, please contact busdev@neb.com.