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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

Catalog # Concentration Size List Price Quantity Your Price
E7335L 96 reactions $608.00
$547.20
E7335S 24 reactions $165.00
$148.50
Catalog # Size List Price Your Price
E7335L 96 reactions $608.00
$547.20
E7335S 24 reactions $165.00
$148.50
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

  • High efficiency adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing

Note: NEBNext® Multiplex Oligos for Illumina® (Index Primer Set 1) (NEB #7335S/L) includes 12 indices. 

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Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER™ Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing.

NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.

Additional single- or dual-barcode primer options are available, in 12- and 96- index formats (NEB #E7500,  NEB #E7710,  NEB #E7730,  NEB #E6609,  NEB #E7600). A methylated version of the NEBNext Adaptor is also available for use with bisulfite sequencing protocols (NEB #E7535).
 

Figure 1: Workflow
Figure 1: Workflow.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  NEBNext Adaptor for Illumina E7337AVIAL -20 1 x 0.24 ml
  USER® Enzyme E7338AVIAL -20 1 x 0.072 ml
  NEBNext Universal PCR Primer for Illumina E6861AVIAL -20 1 x 0.12 ml 10 µM
  NEBNext Index 1 Primer for Illumina E7311AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 2 Primer for Illumina E7312AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 3 Primer for Illumina E7313AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 4 Primer for Illumina E7314AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 5 Primer for Illumina E7315AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 6 Primer for Illumina E7316AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 7 Primer for Illumina E7317AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 8 Primer for Illumina E7318AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 9 Primer for Illumina E7319AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 10 Primer for Illumina E7320AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 11 Primer for Illumina E7321AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Index 12 Primer for Illumina E7322AVIAL -20 1 x 0.01 ml 10 µM
  NEBNext Adaptor for Illumina E7337AAVIAL -20 1 x 0.96 ml
  USER® Enzyme E7338AAVIAL -20 1 x 0.288 ml
  NEBNext Universal PCR Primer for Illumina E6861AAVIAL -20 1 x 0.48 ml 10 µM
  NEBNext Index 1 Primer for Illumina E7311AAVIAL -20 1 x 0.04 ml 10 µM
  NEBNext Index 2 Primer for Illumina E7312AAVIAL -20 1 x 0.04 ml 10 µM
  NEBNext Index 3 Primer for Illumina E7313AAVIAL -20 1 x 0.04 ml 10 µM
  NEBNext Index 4 Primer for Illumina E7314AAVIAL -20 1 x 0.04 ml 10 µM
  NEBNext Index 5 Primer for Illumina E7315AAVIAL -20 1 x 0.04 ml 10 µM
  NEBNext Index 6 Primer for Illumina E7316AAVIAL -20 1 x 0.04 ml 10 µM
  NEBNext Index 7 Primer for Illumina E7317AAVIAL -20 1 x 0.04 ml 10 µM
  NEBNext Index 8 Primer for Illumina E7318AAVIAL -20 1 x 0.04 ml 10 µM
  NEBNext Index 9 Primer for Illumina E7319AAVIAL -20 1 x 0.04 ml 10 µM
  NEBNext Index 10 Primer for Illumina E7320AAVIAL -20 1 x 0.04 ml 10 µM
  NEBNext Index 11 Primer for Illumina E7321AAVIAL -20 1 x 0.04 ml 10 µM
  NEBNext Index 12 Primer for Illumina E7322AAVIAL -20 1 x 0.04 ml 10 µM

Properties & Usage

Materials Required but not Supplied

For the list of additional materials required, please check the manual for your NEBNext Library Prep Kit.


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The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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