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NEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

Catalog # Concentration Size List Price Quantity Your Price
E7535G 6 reactions
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E7535L 96 reactions
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E7535S 24 reactions
Please Inquire
Catalog # Size List Price Your Price
E7535G 6 reactions
E7535L 96 reactions
E7535S 24 reactions
Please Inquire
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

NEBNext® Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina® library production. The unique hairpin loop structure of the NEBNext adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification. This kit includes a methylated version of the NEBNext adaptor, which is compatible with library preparation for bisulfite sequencing protocols.

  • High efficiency adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing
  • Compatible with bisulfite sequencing protocols



Incorporating a novel hairpin loop structure, the NEBNext adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER® Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing.

NEBNext oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.

Properties & Usage

Materials Required but not Supplied

Compatible with the following library preparation kits:
NEBNext Ultra™ II DNA Library Prep Kit for Illumina (#E7645),
NEBNext Ultra™ DNA Library Prep Kit for Illumina (#E7370),
NEBNext DNA Library Prep Master Mix Set for Illumina (#E6040), or
NEBNext DNA Library Prep Reagent Set for Illumina (#E6000)

NEB EpiMark® Bisulfite Conversion Kit (NEB # E3318S)
NEB EpiMark Hot Start Taq DNA Polymerase (NEB # M0490S)

10 mM dNTPs
Freshly prepared 80% Ethanol
Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)
DNA LoBind® Tubes (Eppendorf®)/PCR Plates
Agencourt® AMPure® XP Beads (Beckman Coulter, Inc. #A63881)
Bioanalyzer® (Agilent® Technologies, Inc.)
Nuclease Free Water
0.1X TE (or 10 mM Tris-HCl, pH 7.5-8.0)

Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.NEW ENGLAND BIOLABS®, NEBNEXT® and EPIMARK®are registered trademarks of New England Biolabs, Inc.
NEBNEXT® Adaptors are protected under a pending patent.
USER™ and ULTRA™ are trademarks of New England Biolabs, Inc. 
USER™ is protected by U.S. patent no. 7,435,572 (New England Biolabs, Inc.)

AGENCOURT® and AMPURE® are registered trademarks of Beckman Coulter, Inc.
AGILENT® and BIOANALYZER® are registered trademarks of Agilent Technologies, Inc.
EPPENDORF® and LOBIND® are registered trademarks of Eppendorf AG.
ILLUMINA® is a registered trademark of Illumina, Inc.
QIAQUICK® and MINELUTE® are registered trademarks of Qiagen.

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