NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification. This kit includes 8 i5 index primers and 12 i7 index primers for dual indexing.
For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
E7600S | -20 |
NEBNext Adaptor for Illumina | E7601AVIAL | -20 | 1 x 0.96 ml | ||
USER® Enzyme | E7602AVIAL | -20 | 1 x 0.288 ml | ||
NEBNext i501 Primer | E7603AVIAL | -20 | 1 x 0.06 ml | 10 µM | |
NEBNext i502 Primer | E7604AVIAL | -20 | 1 x 0.06 ml | 10 µM | |
NEBNext i503 Primer | E7605AVIAL | -20 | 1 x 0.06 ml | 10 µM | |
NEBNext i504 Primer | E7606AVIAL | -20 | 1 x 0.06 ml | 10 µM | |
NEBNext i505 Primer | E7607AVIAL | -20 | 1 x 0.06 ml | 10 µM | |
NEBNext i506 Primer | E7608AVIAL | -20 | 1 x 0.06 ml | 10 µM | |
NEBNext i507 Primer | E7609AVIAL | -20 | 1 x 0.06 ml | 10 µM | |
NEBNext i508 Primer | E7610AVIAL | -20 | 1 x 0.06 ml | 10 µM | |
NEBNext i701 Primer | E7611AVIAL | -20 | 1 x 0.04 ml | 10 µM | |
NEBNext i702 Primer | E7612AVIAL | -20 | 1 x 0.04 ml | 10 µM | |
NEBNext i703 Primer | E7613AVIAL | -20 | 1 x 0.04 ml | 10 µM | |
NEBNext i704 Primer | E7614AVIAL | -20 | 1 x 0.04 ml | 10 µM | |
NEBNext i705 Primer | E7615AVIAL | -20 | 1 x 0.04 ml | 10 µM | |
NEBNext i706 Primer | E7616AVIAL | -20 | 1 x 0.04 ml | 10 µM | |
NEBNext i707 Primer | E7617AVIAL | -20 | 1 x 0.04 ml | 10 µM | |
NEBNext i708 Primer | E7618AVIAL | -20 | 1 x 0.04 ml | 10 µM | |
NEBNext i709 Primer | E7619AVIAL | -20 | 1 x 0.04 ml | 10 µM | |
NEBNext i710 Primer | E7620AVIAL | -20 | 1 x 0.04 ml | 10 µM | |
NEBNext i711 Primer | E7621AVIAL | -20 | 1 x 0.04 ml | 10 µM | |
NEBNext i712 Primer | E7622AVIAL | -20 | 1 x 0.04 ml | 10 µM |
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