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NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

Catalog # Concentration Size List Price Quantity Your Price
E6111L 100 reactions $1,798.00
$1,618.20
E6111S 20 reactions $450.00
$405.00
Catalog # Size List Price Your Price
E6111L 100 reactions $1,798.00
$1,618.20
E6111S 20 reactions $450.00
$405.00
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
This module is part of the non-directional Ultra™ II RNA workflow, for Illumina®- compatible non-strand-specific RNA library construction. This workflow is compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enables high yield preparation of high quality libraries from 10 ng – 1 µg total RNA.

Kits are also available that include reagents for every step in the non-directional Ultra II RNA library construction workflow (NEBNext® Ultra II RNA Library Prep Kit for Illumina, NEB #E7770 and NEBNext Ultra II RNA Library Prep with Sample Purification Beads, NEB #E7775).


The NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module has been optimized to generate double-stranded cDNA from first-strand cDNA, as part of the NEBNext non-directional RNA library preparation workflow.

The NEBNExt Ultra II Directional RNA Second Strand Synthesis Module is Designed for Use with the Following:

 

 

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  NEBNext® Second Strand Synthesis Reaction Buffer E6113AVIAL -20 1 x 0.16 ml
  NEBNext® Second Strand Synthesis Enzyme Mix E6112AVIAL -20 1 x 0.08 ml
  NEBNext® Second Strand Synthesis Reaction Buffer E6113AAVIAL -20 1 x 0.8 ml
  NEBNext® Second Strand Synthesis Enzyme Mix E6112AAVIAL -20 1 x 0.4 ml
Application Features
  • RNA Sample Preparation
  • Second Strand Synthesis of cDNA

Properties & Usage

Materials Required but not Supplied

Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)

Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.Phusion® is a registered trademark and property of Thermo Fisher Scientific.

Notice to Customer: Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific.

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