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NEBNext® Ultra™ II RNA First Strand Synthesis Module

Catalog # Concentration Size List Price Quantity Your Price
E7771L 96 reactions $752.00
$676.80
E7771S 24 reactions $235.00
$211.50
Catalog # Size List Price Your Price
E7771L 96 reactions $752.00
$676.80
E7771S 24 reactions $235.00
$211.50
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

This module is part of the Ultra II Directional RNA and Ultra II RNA workflows, for Illumina®- compatible RNA library construction. These workflows are compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enable high yield preparation of high quality libraries from 10 ng – 1 µg total RNA.

 

Kits are also available that include reagents for every step in the Ultra II Directional RNA library construction workflow (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760) and NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads (NEB #E7765)) and non-directional Ultra II RNA library construction workflow (NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770) and NEBNext Ultra II RNA Library Prep with Sample Purification Beads (NEB #E7775)).


The NEBNext Ultra II RNA First Strand Synthesis Module has been optimized to convert a broad range of input amounts of RNA into cDNA using random priming.

The module is optimized for use with the NEBNext Ultra II Directional RNA Second Strand Synthesis Module (NEB #E7550) or the NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (NEB #E6111), and is part of the Ultra II RNA directional or non-directional workflows. These workflows are compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enable high yield preparation of high quality libraries from 10 ng – 1 µg total RNA.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  NEBNext First Strand Synthesis Reaction Buffer E7421AVIAL -20 1 x 0.192 ml
  Random Primers E7422AVIAL -20 1 x 0.048 ml
  NEBNext First Strand Synthesis Enzyme Mix E7761AVIAL -20 1 x 0.048 ml
  NEBNext Strand Specificity Reagent E7766AVIAL -20 1 x 0.192 ml
  NEBNext First Strand Synthesis Reaction Buffer E7421AAVIAL -20 1 x 0.768 ml
  Random Primers E7422AAVIAL -20 1 x 0.192 ml
  NEBNext First Strand Synthesis Enzyme Mix E7761AAVIAL -20 1 x 0.192 ml
  NEBNext Strand Specificity Reagent E7766AAVIAL -20 1 x 0.768 ml
NEBNext Ultra II RNA First Strand Synthesis Module | New England Biolabs
Home Next Generation Sequencing Library Preparation Products NEBNext® Ultra™ II RNA First Strand Synthesis Module

NEBNext® Ultra™ II RNA First Strand Synthesis Module

This module is part of the Ultra II Directional RNA and Ultra II RNA workflows, for Illumina®- compatible RNA library construction. These workflows are compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enable high yield preparation of high quality libraries from 10 ng – 1 µg total RNA.

 

Kits are also available that include reagents for every step in the Ultra II Directional RNA library construction workflow (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB https://www.neb.com/en/#E7760) and NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads (NEB https://www.neb.com/en/#E7765)) and non-directional Ultra II RNA library construction workflow (NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB https://www.neb.com/en/#E7770) and NEBNext Ultra II RNA Library Prep with Sample Purification Beads (NEB https://www.neb.com/en/#E7775)).

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Catalog https://www.neb.com/en/# Concentration Size
E7771S Not Applicable 24 reactions
E7771L Not Applicable 96 reactions
Please enter a quantity for at least one size
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Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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