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NEBNext® Ultra™ End Repair/dA-Tailing Module

Catalog # Concentration Size List Price Quantity Your Price
E7442L 96 reactions
Please Inquire
E7442S 24 reactions
Please Inquire
Catalog # Size List Price Your Price
E7442L 96 reactions
E7442S 24 reactions
Please Inquire
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
This module is part of the original Ultra™ workflow, and is optimized for use with the NEBNext® Ultra Ligation Module (NEB #E7445), for Illumina®-compatible library construction. The original Ultra workflow enables library preparation from 5 ng – 1 μg of input DNA in a streamlined protocol. 

However, we now recommend use of the more recent NEBNext Ultra II DNA workflow, which has been further optimized to provide increased yields of high quality libraries from a broader range of input amounts (500 pg – 1 μg), and a NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546) is available.






 

The NEBNext® Ultra™ End Repair/dA-Tailing Module is optimized to convert 5 ng–1 μg of fragmented DNA to repaired DNA having 5' phosphorylated, 3’ dA-tailed ends. The module is optimized for use with NEBNext Ultra Ligation Module (NEB #E7445), and is part of the original Ultra workflow, which enables library preparation from 5 ng – 1 μg of input DNA in a streamlined protocol. 

Properties & Usage

Materials Required but not Supplied

Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)

Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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