This module is part of the NEBNext® Ultra™ II FS workflow, and is optimized for use with the NEBNext Ultra II Ligation Module (NEB #E7595), for Illumina®-compatible library construction.
Kits that include reagents for every step in the Ultra II FS DNA library construction workflow are also available (NEBNext Ultra II FS DNA Library Prep Kit for Illumina NEB #E7805 and NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads NEB #E6177).
Note: The Ultra II FS (Fragmentation System) enzyme mix does not include NEBNext dsDNA Fragmentase®.
The NEBNext Ultra II FS DNA Module has been optimized to convert 100 pg - 0.5 μg of intact DNA to fragmented, end-repaired DNA having 5´ phosphorylated, 3´ dA-tailed ends. The module is optimized for use with the NEBNext Ultra II Ligation Module (NEB #E7595), and is part of the Ultra II FS DNA workflow.
For Illumina library construction, the NEBNext Ultra II FS DNA Module is designed for use with the following:
For use with NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1) (NEB #E7395), refer to the Protocols tab for UMI Adaptors-specific guidance.
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.