NEBNext® is now available for single cell and ultra-low RNA inputs!
This unique workflow meets the demand for a highly sensitive, yet robust method that consistently enables generation of high quality sequencing data from single cell or ultra-low input RNA.
The NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® uses a template switching method to generate full length cDNAs directly from single cells or 2 pg – 200 ng RNA, followed by conversion to sequence-ready libraries using the Ultra™ II FS workflow. This unique workflow enables generation of the highest yields from a broad range of inputs, and superior transcript detection, while providing reliably consistent performance.
Please note that NEBNext Multiplex Oligos (Adaptors & Primers) are not included in the kit and are available separately.
Features:
Download extensive performance data in our technical note.
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
E6420S | -20 |
NEBNext® Single Cell RT Primer Mix | E6422AVIAL | -20 | 1 x 0.048 ml | ||
NEBNext® Single Cell RT Buffer | E6423AVIAL | -20 | 1 x 0.12 ml | ||
NEBNext® Template Switching Oligo | E6424AVIAL | -20 | 1 x 0.024 ml | ||
NEBNext® Single Cell RT Enzyme Mix | E6425AVIAL | -20 | 1 x 0.048 ml | ||
NEBNext® Single Cell cDNA PCR Master Mix | E6426AVIAL | -20 | 1 x 1.2 ml | ||
NEBNext® Single Cell cDNA PCR Primer | E6427AVIAL | -20 | 1 x 0.048 ml | ||
NEBNext® Cell Lysis Buffer | E6428AVIAL | -20 | 1 x 0.048 ml | ||
Murine RNase Inhibitor | E6429AVIAL | -20 | 1 x 0.024 ml | ||
NEBNext® Bead Reconstitution Buffer | E6430AVIAL | -20 | 1 x 1.08 ml | ||
NEBNext® Adaptor Dilution Buffer | E6431AVIAL | -20 | 1 x 0.576 ml | ||
TE Buffer | E6432AVIAL | -20 | 1 x 1.2 ml | ||
Nuclease-free Water | E6433AVIAL | -20 | 1 x 1.44 ml | ||
NEBNext® Ligation Enhancer | E7374AVIAL | -20 | 1 x 0.024 ml | ||
NEBNext® Ultra™ II Ligation Master Mix | E7648AVIAL | -20 | 1 x 0.72 ml | ||
NEBNext® Ultra II Q5® Master Mix | E7649AVIAL | -20 | 1 x 0.6 ml | ||
NEBNext® Ultra™ II FS Enzyme Mix | E7806AVIAL | -20 | 1 x 0.048 ml | ||
NEBNext® Ultra™ II FS Reaction Buffer | E7807AVIAL | -20 | 1 x 0.168 ml |
E6420L | -20 |
NEBNext® Single Cell RT Primer Mix | E6422AAVIAL | -20 | 1 x 0.192 ml | ||
NEBNext® Single Cell RT Buffer | E6423AAVIAL | -20 | 1 x 0.48 ml | ||
NEBNext® Template Switching Oligo | E6424AAVIAL | -20 | 1 x 0.096 ml | ||
NEBNext® Single Cell RT Enzyme Mix | E6425AAVIAL | -20 | 1 x 0.192 ml | ||
NEBNext® Single Cell cDNA PCR Master Mix | E6426AAVIAL | -20 | 1 x 4.8 ml | ||
NEBNext® Single Cell cDNA PCR Primer | E6427AAVIAL | -20 | 1 x 0.192 ml | ||
NEBNext® Cell Lysis Buffer | E6428AAVIAL | -20 | 1 x 0.192 ml | ||
Murine RNase Inhibitor | E6429AAVIAL | -20 | 1 x 0.096 ml | ||
NEBNext® Bead Reconstitution Buffer | E6430AAVIAL | -20 | 1 x 4.32 ml | ||
NEBNext® Adaptor Dilution Buffer | E6431AAVIAL | -20 | 1 x 2.31 ml | ||
TE Buffer | E6432AAVIAL | -20 | 1 x 4.8 ml | ||
Nuclease-free Water | E6433AAVIAL | -20 | 1 x 5.76 ml | ||
NEBNext® Ligation Enhancer | E7374AAVIAL | -20 | 1 x 0.096 ml | ||
NEBNext® Ultra™ II Ligation Master Mix | E7648AAVIAL | -20 | 3 x 0.96 ml | ||
NEBNext® Ultra II Q5® Master Mix | E7649AAVIAL | -20 | 2 x 1.2 ml | ||
NEBNext® Ultra™ II FS Enzyme Mix | E7806AAVIAL | -20 | 1 x 0.192 ml | ||
NEBNext® Ultra™ II FS Reaction Buffer | E7807AAVIAL | -20 | 1 x 0.672 ml |
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows.
For additional information or to inquire about commercial use, please contact busdev@neb.com.