pBR322 DNA-MspI Digest

Catalog #	Concentration	Size	List Price	Quantity	Your Price
N3032S	1000 µg/ml	50 gel lanes	$122.00		$109.80

Catalog #	Size	List Price	Your Price
N3032S	50 gel lanes	$122.00	$109.80

The MspI digest of pBR322 DNA yields 26 fragments. Suitable for use as molecular weight standards for agarose gel electrophoresis (1,2).

Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.

Recommended gel percentage range: 2-3.5%
Optimum separation on 3%

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

N3032S -20

	pBR322 DNA-MspI Digest	N3032SVIAL	-20	1 x 0.05 ml	1 mg/ml
	Gel Loading Dye, Purple (6X), no SDS	B7025SVIAL	25	1 x 1 ml	6 X

Properties & Usage

Bases

Fragment	Mass (ng)	bp
1	143	622
2	121	527
3	93	404
4	70	307
5	55	242
6	55	238
7	50	217
8	46	201
9	44	190
10	41	180
11	74	160/160
12	68	147/147
13	28	123
14	25	110
15	21	90
16	17	76
17	15	67
18	16	34/34
19	12	26/26
20	3	15
21	4	9/9

Effective Size Range

9bp to 622bp

Storage Buffer

10 mM Tris-HCl
1 mM EDTA
pH 8 @ 25°C

Companion Products

Materials Sold Separately

Gel Loading Dye, Purple (6X), no SDS

Notes

Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH₂O.

1X Gel Loading Dye, Purple, no SDS:
2.5% Ficoll®-400
10mM EDTA
3.3mM Tris-HCl (pH 8.0@25°C)
0.02% Dye 1
0.001% Dye 2

References

Sutcliffe, J.G. (1978). Cold Spring Harbor Symp. Quant. Bio.. 43, 77-90.
Peden, K.W.C. (1983). Gene. 22, 277-280.

Protocols

Suggested Loading Protocol for DNA Ladders & Markers

FAQs

Tech Tips

To make it ready-to-load, dilute in TE buffer instead of water.

Citations

See more details on Bioz

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

N3032S_L_v1

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.