Monarch® Plasmid Miniprep Kit

Catalog #	Size	List Price	Your Price
T1010L	250 preps
T1010S	50 preps

The Monarch Plasmid Miniprep Kit is a rapid and reliable method for the purification of high quality plasmid DNA. This method employs standard cell resuspension, alkaline lysis, and neutralization steps, with the additional benefit of color indicators at certain steps to easily monitor completion. Unique wash buffers ensure salts, proteins, RNA and other cellular components are removed, allowing low-volume elution of concentrated, highly pure DNA. Protocols are fast and user friendly, saving you valuable time. Elution in as little as 30 μl provides concentrated DNA for use in downstream applications, such as restriction digests, DNA sequencing, PCR and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.

View our videos on protocols, tips, and recycling Monarch.

Advantages:

Elute in as little as 30 μl
Prevent buffer retention and salt carry-over with optimized column design
Reduce hands on time with faster protocols and less spin time
Monitor completion of certain steps using colored buffer system
No need to add RNase before starting
Easily label columns using tab and frosted surfaces
Buffers and columns available separately
Significantly less plastic used when compared with other kits
Responsibly-sourced and recyclable packaging
No hazardous materials fees

Specifications

Culture Volume:	1-5 ml, not to exceed 15 OD units
Binding Capacity:	up to 20 μg
Plasmid Size:	up to 25 kb
Typical Recovery:	up to 20 μg. Yield depends on plasmid copy number, host strain, culture volume, and growth conditions.
Elution Volume:	≥ 30 μl
Purity:	A_260/280 and A_260/230 ≥ 1.8
Protocol Time:	10½ minutes of spin and incubation time
Compatible Downstream Applications:	restriction digestion and other enzymatic manipulations, transformation, transfection, DNA sequencing, PCR, labeling, cell-free protein synthesis, etc.

Monarch Plasmid Miniprep Kits consistently produce more concentrated plasmid DNA with equivalent yield, purity and functionality as compared to the leading supplier. Preps were performed according to recommended protocols using 1.5 ml aliquots of the same overnight culture. One microliter of each prep was digested with HindIII-HF (NEB #R3104) to linearize the vector and the digests were resolved on a 1% w/v agarose gel. — Preps were performed according to recommended protocols using 1.5 ml aliquots of the same overnight culture. One microliter of each prep was digested with HindIII-HF (NEB #R3104) to linearize the vector and the digests were resolved on a 1% w/v agarose gel.

Plasmid DNA purified using the Monarch Plasmid Miniprep Kit produces transfection efficiencies equivalent to or better than plasmid DNA purified using the Qiagen QIAprep® Spin Miniprep Kit. Plasmid DNA encoding constitutively expressed GFP (pEGFP-C2) was prepared using either Monarch Plasmid Miniprep Kit or Qiagen QIAprep Spin Miniprep Kit. Four different cell lines (Cos-7, HEK293, HeLa, and Huh-7) were grown to 80-90% confluence and transfected with 100 ng of each plasmid, in complex with 0.3 μl Lipofectamine 2000, and 10 μl Opti-MEM. Five replicates for each cell type were performed using both DNA preps. GFP expressing cells were counted by flow cytometry 48 hrs post-transfection with a minimum of 2000 events collected per well. Average percentage of cells expressing GFP from all replicates is graphed and used as a measure of transfection efficiency. — Plasmid DNA encoding constitutively expressed GFP (pEGFP-C2) was prepared using either Monarch Plasmid Miniprep Kit or Qiagen QIAprep Spin Miniprep Kit. Four different cell lines (Cos-7, HEK293, HeLa, and Huh-7) were grown to 80-90% confluence and transfected with 100 ng of each plasmid, in complex with 0.3 μl Lipofectamine 2000, and 10 μl Opti-MEM. Five replicates for each cell type were performed using both DNA preps. GFP expressing cells were counted by flow cytometry 48 hrs post-transfection with a minimum of 2000 events collected per well. Average percentage of cells expressing GFP from all replicates is graphed and used as a measure of transfection efficiency.

Features

Elute in as little as 30 μl
Prevent buffer retention and salt carry-over with optimized column design
Reduce hands on time with faster protocols and less spin time
Monitor completion of certain steps using colored buffer system
No need to add RNase before starting
Easily label columns using tab and frosted surfaces
Buffers and columns available separately
Significantly less plastic used when compared with other kits
Responsibly-sourced and recyclable packaging
No hazardous materials fees

Properties & Usage

Companion Products

Materials Sold Separately

Notes

The kit should be stored at room temperature. Always keep buffer bottles tightly closed and keep columns sealed in the enclosed zip-lock bag. After Plasmid Neutralization Buffer (B3) is opened, it should be stored at 4°C. For information regarding the composition of buffers, please consult the Safety Data Sheets. Proper laboratory safety practices should be employed, including the use of lab coats, gloves, and eye protection.

Protocols

Usage Guidelines

DataCards

manualT1010

FAQs

Troubleshooting

Troubleshooting Guide for DNA Cleanup and Plasmid Purification

Tech Tips

Citations

Additional Citations

Fomenkov, A., Vincze, T., Mersha, F., Roberts, R.J. (2018) Complete genome sequence and methylome analysis of Bacillus caldolyticus NEB414. Genome Announ. 6 (6), e01605-17.PubMedID: 29439055 , DOI: 10.1128/genomeA.01605-17
Bornikoel J, Staiger J, Madlung J, Forchhammer K, Maldener I. (2018) LytM factor Alr3353 affects filament morphology and cell-cell communication in the multicellular cyanobacterium Anabaena sp. PCC 7120 Mol Microbiol PubMedID: 29437253,
Kanpiengjai A, Nguyen TH, Haltrich D, Khanongnuch C. (2017) Expression and comparative characterization of complete and C-terminally truncated forms of saccharifying a-amylase from Lactobacillus plantarum S21. Int J Biol MacromolPubMedID: 28587961
Wei, H., Yan, B., Gagneur, J., Conradt, B. (2017) Caenorhabditis elegans CES-1 snail represses Pig-1 MELK expression to control asymmetric cell division. Genetics 206(4),PubMedID: 28652378
Luck A, Yuan X2, Voronin D, Slatko B, Hamza I, Foster JM4. (2016) Heme acquisition in the parasitic filarial nematode Brugia malayi FASEB JPubMedID: 27363426

Publications

Wei, H., Yan, B., Gagneur, J., Conradt, B. (2017). Caenorhabditis elegans CES-1 snail represses Pig-1 MELK expression to control asymmetric cell division. Genetics. 206(4),PubMedID: 28652378
Vladimir Potapov, Jennifer L. Ong. (2017). Examining Sources of Error in PCR by Single-Molecule Sequencing. PLOS One.PubMedID: 28683110

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Legal And Disclaimer

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.