Monarch® DNA Gel Extraction Kit

Catalog #	Size	List Price	Your Price
T1020L	250 preps
T1020S	50 preps	$152.00	$136.80

The Monarch DNA Gel Extraction Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from agarose gels. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Unlike other kits, there is no need to add isopropanol to the melted agarose prior to loading on the column, saving you a step. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging

View our videos on protocols, tips, and recycling Monarch.

Monarch columns are designed for performance. Monarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification. — Monarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification.

Advantages:

Elute in as little as 6 μl
Prevent buffer retention and salt carry-over with optimized column design
Save time with fast, user-friendly protocols
No need to monitor pH or add isopropanol
Buffers and columns available separately
Significantly less plastic used when compared with other kits
Responsibly-sourced and recyclable packaging

Specifications

DNA Sample Type:	double-stranded DNA from agarose gels
Binding Capacity:	up to 5 μg
DNA Size Range:	~50 bp to 25 kb
Typical Recovery:	DNA (50 bp to 10 kb): 70–90% DNA (11–23 kb): 50–70%
Elution Volume:	≥ 6 μl
Purity:	A_260/280 > 1.8
Protocol Time:	10 minutes of spin and incubation time
Compatible Downstream Applications:	ligation, restriction digestion, labeling and other enzymatic manipulations, library construction and DNA sequencing.

DNA purified from the Monarch DNA Gel Extraction Kit is recovered with similar efficiency and purity as the leading supplier, but is more highly concentrated, facilitating its use in downstream applications. One microgram aliquots of a 3 kb fragment were resolved on a 1% w/v agarose gel, excised, and processed with different kits using manufacturer-specified minimum elution volumes. Values reported are the concentration and purity data determined by Nanodrop™ readings, as well as recovery calculations based on the eluted DNA concentration and recovered volume. — One microgram aliquots of a 3 kb fragment were resolved on a 1% w/v agarose gel, excised, and processed with different kits using manufacturer-specified minimum elution volumes. Values reported are the concentration and purity data determined by Nanodrop™ readings, as well as recovery calculations based on the eluted DNA concentration and recovered volume.

Monarch DNA Gel Extraction Kit reproducibly recovers DNA over a broad range of molecular weights. A mixture of 7 DNA fragments ranging from 10 kb down to 0.5 kb was prepared and one-half of the mixture was resolved on a 1% gel. Each fragment was manually excised from the agarose gel and processed using the Monarch DNA Gel Extraction Kit. The entire elution of each fragment was resolved on a new gel with the remainder of the original mixture for comparison. — A mixture of 7 DNA fragments ranging from 10 kb down to 0.5 kb was prepared and one-half of the mixture was resolved on a 1% gel. Each fragment was manually excised from the agarose gel and processed using the Monarch DNA Gel Extraction Kit. The entire elution of each fragment was resolved on a new gel with the remainder of the original mixture for comparison.

DNA purified from agarose gels using the Monarch DNA Gel Extraction Kit can be reproducibly isolated and ligated. Two micrograms of a 3 kb fragment with compatible ends was resolved on a 1% agarose gel, excised, and purified using the Monarch DNA Gel Extraction Kit. Samples were eluted in 20 μl and a fraction (1/4 th of total) was ligated using the Blunt/TA Ligase Master Mix (NEB #M0367 (https://www.neb.com/products/m0367-blunt-ta-ligase-master-mix) ). Representative samples from 5 replicates were resolved on a second 1% agarose gel. M is the 1 kb DNA Ladder (NEB #N3232 (https://www.neb.com/products/n3232-1-kb-dna-ladder) ). — Two micrograms of a 3 kb fragment with compatible ends was resolved on a 1% agarose gel, excised, and purified using the Monarch DNA Gel Extraction Kit. Samples were eluted in 20 μl and a fraction (1/4 th of total) was ligated using the Blunt/TA Ligase Master Mix (NEB #M0367). Representative samples from 5 replicates were resolved on a second 1% agarose gel. M is the 1 kb DNA Ladder (NEB #N3232).

Features

Elute in as little as 6 μl
Prevent buffer retention and salt carry-over with optimized column design
Save time with fast, user-friendly protocols
No need to monitor pH or add isopropanol
Buffers and columns available separately
Significantly less plastic used when compared with other kits
Responsibly-sourced and recyclable packaging

Properties & Usage

Companion Products

Materials Sold Separately

Notes

The kit should be stored at room temperature. Always keep buffer bottles tightly closed and keep columns sealed in the enclosed zip-lock bag. For information regarding the composition of buffers, please consult the Safety Data Sheets. Proper laboratory safety practices should be employed, including the use of lab coats, gloves and eye protection.

Protocols

Monarch® DNA Gel Extraction Kit Protocol (NEB #T1020)

Usage Guidelines

DataCards

manualT1020

FAQs

Troubleshooting

Troubleshooting Guide for DNA Cleanup and Plasmid Purification

Tech Tips

Citations

Additional Citations

Wang M, Wang B, Jiang K, Liu M, Shi X, Wang L. (2018) A mitochondrial manganese superoxide dismutase involved in innate immunity is essential for the survival of Chlamys farreri Fish Shellfish Immunol PubMedID: 29127027,
Kose, S. H., Grice, K., Orsi, W. D., Ballal, M., and Coolen, M. J. L. (2018) Metagenomics of pigmented and cholesterol gallstones: the putative role of bacteria Sci Rep
Bornikoel J, Staiger J, Madlung J, Forchhammer K, Maldener I. (2018) LytM factor Alr3353 affects filament morphology and cell-cell communication in the multicellular cyanobacterium Anabaena sp. PCC 7120 Mol Microbiol PubMedID: 29437253,
Kang B, Peng B, Wu H, Liu L, Wu W, Gu Q. (2018) Host-associated selection of a P3 mutant of zucchini yellow mosaic virus affects viral infectivity in watermelon Arch Virol PubMedID: 29426994,
Perniss A, Schmidt N, Gurtner C, Dietert K, Schwengers O, Weigel M, Hempe J, Ewers C, Pfeil U, Gärtner U, Gruber AD, Hain T, Kummer W (2018) Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice Sci Rep 8(1):5681, DOI: 10.1038/s41598-018-23830-4
Roussy M, Bilodeau M, Jouan L, Tibout P, Laramée L, Lemyre E, Cardin S, Sauvageau C, Couture F, Choblet A, Patey N, Gendron P, Duval M, Teira P, Hébert J, Wilhelm BT, Choi JK, Gruber TA, Bittencourt H, Cellot S. (2018) NUP98-BPTF gene fusion identified in primary refractory acute megakaryoblastic leukemia of infancy Genes Chromosomes Cancer PubMedID: 29427526,
Hua Yang, Silvia Jenni, Milena Colovic, Helen Merkens, Carlee Poleschuk, Isabel Rodrigo, Qing Miao, Bruce F. Johnson4, Michael J. Rishel4, Vesna Sossi, Jack M. Webster, François Bénard and Paul Schaffer (2017) F-5-Fluoroaminosuberic Acid as a Potential Tracer to Gauge Oxidative Stress in Breast Cancer Models. J Nucl MedPubMedID: 5331935
Zhang Y, Tanner N. (2017) Isothermal Amplification of Long, Discrete DNA Fragments Facilitated by Single-Stranded Binding Protein BioTechniques 1–8,PubMedID: 28819114, DOI: 10.2144/btn-2024-0012
Adam Waalkes, Kelsi Penewit, Brent L. Wood, David Wu, and Stephen J. Salipante (2017) Ultrasensitive detection of acute myeloid leukemia minimal residual disease using single molecule molecular inversion probes. HaematologicaPubMedID: 28572161
Hannah G. Reich, Deborah L. Robertson, Gretchen Goodbody-Gringley (2016) Do the shuffle: Changes in Symbiodinium consortia throughout juvenile coral development. PLOS OnePubMedID: 28182684

Publications

Zhang Y, Tanner N. (2017). Isothermal Amplification of Long, Discrete DNA Fragments Facilitated by Single-Stranded Binding Protein BioTechniques. 1–8,PubMedID: 28819114, DOI: 10.2144/btn-2024-0012

Quality Control Assay

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Specifications

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Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

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