100 bp DNA Ladder

Catalog #	Concentration	Size	List Price	Quantity	Your Price
N3231L	500 µg/ml	500 gel lanes	$364.00		$327.60
N3231S	500 µg/ml	100 gel lanes	$94.00		$84.60

Catalog #	Size	List Price	Your Price
N3231L	500 gel lanes	$364.00	$327.60
N3231S	100 gel lanes	$94.00	$84.60

A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100-1,517 base pairs. The 500 and 1,000 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each of the bands is provided (assuming a 0.5 μg load) for approximating the mass of DNA in comparably intense samples of similar size.

Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.

NEB also offers a Quick-Load version of this ladder with purple dye.

Recommended gel percentage range: 1.2-3%
Optimum separation on 2%

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

N3231S -20

	100 bp DNA Ladder	N3231SVIAL	-20	1 x 0.1 ml	500 µg/ml
	Gel Loading Dye, Purple (6X), no SDS	B7025SVIAL	25	1 x 1 ml	6 X

N3231L -20

	100 bp DNA Ladder	N3231LVIAL	-20	1 x 0.5 ml	500 µg/ml
	Gel Loading Dye, Purple (6X), no SDS	B7025SVIAL	25	1 x 1 ml	6 X

Properties & Usage

Bases

Fragment	Mass (ng)	bp
1	45	1517
2	35	1200
3	95	1000
4	27	900
5	24	800
6	21	700
7	18	600
8	97	500/517
9	38	400
10	29	300
11	25	200
12	48	100

Effective Size Range

100bp to 1,517bp

Storage Buffer

10 mM Tris-HCl
1 mM EDTA
pH 8 @ 25°C

Companion Products

Materials Sold Separately

Gel Loading Dye, Purple (6X), no SDS

Notes

This ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
We recommend loading 0.5 μg of 100 bp DNA Ladder diluted in sample buffer.
All fragments have 4-base, 5´ overhangs that can be end labeled using T4 Polynucleotide Kinase (#M0201) or filled-in using DNA Polymerase I, Klenow Fragment (#M0210) (1). Use α-[³²P] dATP or α-[³²P] dTTP for the fill-in reaction.
100 bp DNA Ladder is stable for at least 3 months at 4°C.
For long term storage store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH₂O.
Due to the limitations of the acrylamide gel technology, one or two extra bands may be visible on the DNA ladders when run on a polyacrylamide gel.
1X Gel Loading Dye, Purple (6X), no SDS:
2.5% Ficoll®-400
10mM EDTA
3.3mM Tris-HCl (pH 8.0@25°C)
0.02% Dye 1
0.001% Dye 2

References

Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual (2nd Ed.). 10.51-10.67.

Protocols

Selection Charts

DNA Markers & Ladders Selection Tool

FAQs

Tech Tips

To make it ready-to-load, dilute in TE buffer instead of water.

Citations

See more details on Bioz

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

N3231S_L_v1

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.