Quick-Load® Purple 50 bp DNA Ladder

Catalog #	Concentration	Size	List Price	Quantity	Your Price
N0556S	100 µg/ml	250 gel lanes	$140.00		$126.00

Catalog #	Size	List Price	Your Price
N0556S	250 gel lanes	$140.00	$126.00

Quick-load Purple 50 bp DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing one tracking dye. Tracking dye does not leave a shadow under UV light.

The Quick-Load Purple 50 bp DNA Ladder consists of a proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 17 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 50 to 1,350 base pairs. The 200 and 500 bp bands have increased intensity to serve as reference points.

Recommended gel percentage range: 2-3.5%
Optimum separation on 3%

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

N0556S 4

	Quick-Load^® Purple 50 bp DNA Ladder	N0556SVIAL	4	1 x 1.25 ml	100 µg/ml
	Gel Loading Dye, Purple (6X), no SDS	B7025SVIAL	25	1 x 1 ml	6 X

Properties & Usage

Bases

Fragment	Mass (ng)	bp
1	103	1350
2	70	916
3	58	766
4	54	700
5	50	650
6	46	600
7	42	550
8	76	500
9	34	450
10	31	400
11	27	350
12	46	300
13	57	250
14	107	200
15	46	150
16	69	100
17	84	50

Effective Size Range

50bp to 1,350bp

Storage Buffer

2.5% FicollÂ®-400
10 mM EDTA
3.3 mM Tris-HCl
0.001% Dye 2
0.02% Dye 1
pH 8 @ 25°C

Companion Products

Notes

Preparation of DNA: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
We recommend loading 5–10 μl (0.5–1 μg) of Quick-Load Purple 50 bp DNA Ladder per gel lane. In general, it is recommended to load 1 µl per mm of gel lane.
The Quick-Load Purple 50 bp DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
Quick-Load Purple 50 bp DNA Ladder is stable for at least 6 months at 25°C. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing. If not well mixed, the load may float out of the wells.
1X Gel Loading Dye, Purple, no SDS:
2.5% Ficoll®-400
10 mM EDTA
3.3 mM Tris-HCl (pH 8.0@25°C)
0.02% Dye 1
0.001% Dye 2

References

Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. (2nd ed.), pp.10.51-10.67, Cold Spring Harbor Laboratory Press.

Protocols

Protocol for Quick-Load Purple 50 bp DNA Ladder (NEB #N0556)

Selection Charts

DNA Markers & Ladders Selection Tool

FAQs

Citations

See more details on Bioz

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

N0556S_v1

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.