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Monarch® DNA/RNA Protection Reagent

Catalog # Concentration Size List Price Quantity Your Price
T2011L 56 ml $180.00
$162.00
Catalog # Size List Price Your Price
T2011L 56 ml $180.00
$162.00
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • Preserves and stabilizes RNA and DNA
  • A component of the Monarch® Total RNA Miniprep Kit (NEB #T2010)
  • Supplied as a 2X concentrate
  • Supplied in 2 bottles, each containing 28 ml
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    Monarch® Total RNA Miniprep Protocol
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    Tips for Successful RNA Purification using Monarch® Total RNA Miniprep Kit
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    Avoiding RNase Contamination


Monarch® DNA/RNA Protection Reagent is a component of the Monarch Total RNA Miniprep Kit. It is a solution for the preservation of nucleic acids in various tissue types. It is supplied as a 2X concentrate. This reagent does not need to be removed before RNA extraction with the Monarch Total RNA Miniprep Kit.

Figure 1: Monarch DNA/RNA Protection Reagent preserves RNA integrity for up to 1 month at 4°C


Samples were incubated in Monarch DNA/RNA Protection Reagent (NEB #T2011) at 4°C for the indicated time and subsequently processed using the Monarch Total RNA Miniprep Kit (NEB #T2010). Samples were eluted in 100 µl nuclease-free water. Rat liver and blood samples were diluted 5-fold (HeLa cells were not diluted), and 1 µl of each was loaded onto a Bioanalyzer Nano chip (HeLa and rat liver) or Pico chip (blood). The RIN values demonstrate the ability of the Protection Reagent to preserve RNA integrity over time.

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Monarch® DNA/RNA Protection Reagent T2011-1 25 2 x 28 ml

Properties & Usage



Companion Products
Materials Sold Separately
Protocols
  1. Quick Protocol for Total RNA Extraction (NEB #T2010)
  2. Total RNA Purification from Samples Stored in Monarch DNA/RNA Protection Reagent using the Monarch Total RNA Miniprep Kit (NEB #T2010)
  3. RNA Extraction from Buccal/Nasopharyngeal Swabs Using the Monarch RNA Cleanup Kits
  4. RNA Extraction from Saliva Using the Monarch RNA Cleanup Kits
  5. Automation of Viral RNA Extraction from Saliva Using the Monarch Spin RNA Cleanup Kit
  6. Automation of RNA Extraction from Saliva Using the Monarch Total RNA Miniprep Kit
  7. Protocol for Genomic DNA Extraction from Cultured Cells Stabilized with Monarch® DNA/RNA Protection Reagent (NEB #2011) using the Monarch Spin gDNA Extraction Kit (NEB #T3010)
  8. Protocol for Genomic DNA Extraction from Blood Stabilized with Monarch® DNA/RNA Protection Reagent (NEB #T2011) using the Monarch Spin gDNA Extraction Kit (NEB #T3010)
  9. Protocol for Genomic DNA Extraction from Tissue Samples Stabilized with Monarch® DNA/RNA Protection Reagent (NEB #T2011) using the Monarch Spin gDNA Extraction Kit (NEB #T3010)
Usage Guidelines
DataCards
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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