Monarch^® DNA/RNA Protection Reagent

Product Information

Monarch^® DNA/RNA Protection Reagent is a component of the Monarch Total RNA Miniprep Kit. It is a solution for the preservation of nucleic acids in various tissue types. It is supplied as a 2X concentrate. This reagent does not need to be removed before RNA extraction with the Monarch Total RNA Miniprep Kit.

Figure 1: Monarch DNA/RNA Protection Reagent preserves RNA integrity for up to 1 month at 4°C

This product is related to the following categories:

Total RNA Extraction & Purification Products,

RNA Extraction and Purification,

Nucleic Acid Purification

• Reagents Supplied

  The following reagents are supplied with this product:

  NEB https://www.neb.com/en/#	Component Name	Component https://www.neb.com/en/#	Stored at (°C)	Amount	Concentration
  T2011L     25       Monarch® DNA/RNA Protection Reagent T2011-1 25 2 x 28 ml Not Applicable

• Related Products

  Companion Products

  • Luna^® Universal One-Step RT-qPCR Kit
  • Luna^® Universal Probe One-Step RT-qPCR Kit
  • ProtoScript® II First Strand cDNA Synthesis Kit
  • NEBNext^® Ultra^™ II RNA Library Prep Kit for Illumina^®
  • NEBNext^® Ultra^™ II Directional RNA Library Prep Kit for Illumina^®
  • NEBNext^® Ultra^™ II Directional RNA Library Prep with Sample Purification Beads
  • NEBNext® Poly(A) mRNA Magnetic Isolation Module
  • ProtoScript® II Reverse Transcriptase

  Materials Sold Separately

  • Monarch® RNA Purification Columns
  • Monarch^® Total RNA Miniprep Kit
  • Monarch^® gDNA Removal Columns
  • Monarch^® Collection Tubes II
  • Monarch^® RNA Lysis Buffer
  • Monarch^® RNA Priming Buffer
  • Monarch^® RNA Wash Buffer
  • Monarch^® Total RNA Miniprep Enzyme Pack
  • Nuclease-free Water

Protocols, Manuals & Usage

• Protocols

  1. Quick Protocol for Total RNA Extraction (NEB https://www.neb.com/en/#T2010)
  2. Total RNA Purification from Samples Stored in Monarch DNA/RNA Protection Reagent using the Monarch Total RNA Miniprep Kit (NEB https://www.neb.com/en/#T2010)
  3. RNA Extraction from Buccal/Nasopharyngeal Swabs Using the Monarch RNA Cleanup Kits
  4. RNA Extraction from Saliva Using the Monarch RNA Cleanup Kits
  5. Automation of Viral RNA Extraction from Saliva Using the Monarch Spin RNA Cleanup Kit
  6. Automation of RNA Extraction from Saliva Using the Monarch Total RNA Miniprep Kit
  7. Protocol for Genomic DNA Extraction from Cultured Cells Stabilized with Monarch^® DNA/RNA Protection Reagent (NEB https://www.neb.com/en/#2011) using the Monarch Spin gDNA Extraction Kit (NEB https://www.neb.com/en/#T3010)
  8. Protocol for Genomic DNA Extraction from Blood Stabilized with Monarch^® DNA/RNA Protection Reagent (NEB https://www.neb.com/en/#T2011) using the Monarch Spin gDNA Extraction Kit (NEB https://www.neb.com/en/#T3010)
  9. Protocol for Genomic DNA Extraction from Tissue Samples Stabilized with Monarch^® DNA/RNA Protection Reagent (NEB https://www.neb.com/en/#T2011) using the Monarch Spin gDNA Extraction Kit (NEB https://www.neb.com/en/#T3010)

• Manuals

  The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

  • manualT2010

• Usage Guidelines

  • General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit
  • Guidance on Choosing Sample Input Amounts when Using the Monarch Total RNA Miniprep Kit
  • Guidelines for Purification of RNA from Cultured Mammalian Cells
  • Guidelines for RNA Purification from Bacteria
  • Guidelines for RNA Purification from Leukocytes (WBCs, PBMCs)
  • Guidelines for RNA Purification from Plants
  • Guidelines for RNA Purification from Tissues 
  • Guidelines for RNA Purification from Whole Blood
  • Guidelines for RNA Purification from Yeast
  • Guidelines for Sample Stabilization Using the Monarch DNA/RNA Protection Reagent

• Application Notes

  • Purification of synthetic SARS-CoV-2 viral RNA from biological samples using the Monarch^® Total RNA Miniprep Kit and the Monarch RNA Cleanup Kit

FAQs & Troubleshooting

• FAQs

  1. How can I preserve my sample if I am not ready to extract RNA right after harvest?
  2. Can the Monarch Total RNA Miniprep Kit (NEB https://www.neb.com/en/#T2010) be used to purify RNA from saliva?
  3. Can the Monarch Total RNA Miniprep Kit NEB T2010 be used to purify RNA from buccal cheek swabs?
  4. Can the Monarch Total RNA Miniprep Kit (NEB https://www.neb.com/en/#T2010) be used to purify RNA from plasma or serum?
  5. Can the Monarch Total RNA Miniprep Kit (NEB https://www.neb.com/en/#T2010) be used to purify RNA from nucleated blood (e.g. bird, fish, reptile)?
  6. Can the Monarch Total RNA Miniprep Kit be used to purify RNA from zebrafish embryos/larvae?
  7. Can I lyse my adherent cells directly in their wells when using the Monarch Total RNA Miniprep Kit?
  8. Can extraction of RNA with the Monarch kits be automated?
  9. Is live SARS-CoV-2 virus inactivated during extraction with the Monarch Total RNA Miniprep Kit?
  10. Are the Monarch HMW DNA Extraction kits compatible with samples stored in Monarch DNA/RNA Protection Reagent, RNAlater, DNA/RNA Shield etc.? 
  11. Are samples stored in DNA/RNA Protection Reagent compatible with the new version of the kit (Monarch Spin RNA Isolation Kit (Mini), NEB https://www.neb.com/en/#T2110)?

• Troubleshooting

  • Troubleshooting Guide for Total RNA Extraction & Purification

Citations & Technical Literature

• Citations

  Product Citation Tool

Quality, Safety & Legal

• Quality Control Assays

  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

• Specifications & Change Notifications

  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

  • T2011L_v2

• Certificate of Analysis

  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

  • T2011L_v1_10053791
  • T2011L_v1_10044093
  • T2011L_v1_10053942
  • T2011L_v1_10074652
  • T2011L_v1_10079498
  • T2011L_v2_10097583
  • T2011L_v2_10141099
  • T2011L_v2_10144559
  • T2011L_v2_10144569
  • T2011L_v2_10166736
  • T2011L_v2_10175995
  • T2011L_v2_10244823
  • T2011L_v2_10247130

• Safety Data Sheets

  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

  • Monarch^® DNA/RNA Protection Reagent

• Legal and Disclaimers

  Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.