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NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads

Catalog # Concentration Size List Price Quantity Your Price
E6350L 24 reactions
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E6350S 6 reactions
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E6350X 96 reactions
Please Inquire
Catalog # Size List Price Your Price
E6350L 24 reactions
E6350S 6 reactions
E6350X 96 reactions
Please Inquire
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

The NEBNext rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads employs an RNaseH-based method (1), (2) to deplete both cytoplasmic (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochondrial ribosomal RNA (12S rRNA and 16S rRNA) from human, mouse and rat total RNA preparations. This product is suitable for both intact and degraded RNA (e.g. FFPE RNA). The resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis applications. For added convenience, this kit contains NEBNext RNA Sample Purification Beads (Agencourt® RNAClean® XP beads from Beckman Coulter).

The NEBNext rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads employs an RNaseH-based method (1), (2) to deplete both cytoplasmic (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochondrial ribosomal RNA (12S rRNA and 16S rRNA) from human, mouse and rat total RNA preparations. This product is suitable for both intact and degraded RNA (e.g., FFPE RNA). The resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis applications. For added convenience, this kit contains NEBNext RNA Sample Purification Beads (Agencourt RNAClean XP beads from Beckman Coulter).
 
NEBNext rRNA Depletion Kit Workflow Times
rRNA Depletion

Properties & Usage

Materials Required but not Supplied

  • Magnetic Rack (Alpaqua, Cat. #A001322 or equivalent)
  • Thermal Cycler


References
  • Adiconis, X. et al (2013). Comparative analysis of RNA sequencing methods for degraded or low-input samples. Nature Methods. 10, 623-629.
  • Morlan, J.D. et al. (2012). Selective depletion of RNA enables whole transcriptome profiling of archival fixed tissue. PLos One. 77, e42882.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
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Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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