This kit will be discontinued on December 15, 2024.
An updated version of this kit (NEB #E7400) is available for superior performance.
To request a sample of our NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat), please click here.
Ribosomal RNAs (rRNAs) are extremely abundant, constituting 80–90% of total RNA. Efficient removal of rRNA is critical to enable cost-effective sequencing of RNA samples, but this can be especially challenging with low quality RNA (e.g. FFPE RNA) and with low input amounts. The NEBNext rRNA Depletion kit employs the efficient RNase H method, as well as complete probe tiling of rRNA, thereby ensuring that even degraded rRNA is hybridized and subsequently removed.
The NEBNext rRNA Depletion Kit (Human/Mouse/Rat) employs an RNaseH-based method (1,2) to deplete both cytoplasmic (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochondrial ribosomal RNA (12S rRNA and 16S rRNA) from human, mouse and rat total RNA preparations. This product is suitable for both intact and degraded RNA (e.g. FFPE RNA). The resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis applications.
Now also available with optional Agencourt® RNAClean® XP beads for RNA purification.
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
E6310S | -20 |
RNase H Reaction Buffer | E6312-2VIAL | -20 | 1 x 0.012 ml | ||
NEBNext rRNA Depletion Solution | E6313-2VIAL | -20 | 1 x 0.01 ml | ||
NEBNext Probe Hybridization Buffer | E6314-2VIAL | -20 | 1 x 0.012 ml | ||
DNase I Reaction Buffer | E6315-2VIAL | -20 | 1 x 0.03 ml | ||
DNase I (RNase-free) | E6316-2VIAL | -20 | 1 x 0.015 ml | ||
Nuclease-free Water | E6317-2VIAL | -20 | 1 x 0.4 ml | ||
NEBNext RNase H | E6318-2VIAL | -20 | 1 x 0.012 ml |
E6310L | -20 |
RNase H Reaction Buffer | E6312-3VIAL | -20 | 1 x 0.048 ml | ||
NEBNext rRNA Depletion Solution | E6313-3VIAL | -20 | 1 x 0.024 ml | ||
NEBNext Probe Hybridization Buffer | E6314-3VIAL | -20 | 1 x 0.048 ml | ||
DNase I Reaction Buffer | E6315-3VIAL | -20 | 1 x 0.12 ml | ||
DNase I (RNase-free) | E6316-3VIAL | -20 | 1 x 0.06 ml | ||
Nuclease-free Water | E6317-3VIAL | -20 | 1 x 1.5 ml | ||
NEBNext RNase H | E6318-3VIAL | -20 | 1 x 0.048 ml |
E6310X | -20 |
RNase H Reaction Buffer | E6312-4VIAL | -20 | 1 x 0.192 ml | ||
NEBNext rRNA Depletion Solution | E6313-4VIAL | -20 | 1 x 0.096 ml | ||
NEBNext Probe Hybridization Buffer | E6314-4VIAL | -20 | 1 x 0.192 ml | ||
DNase I Reaction Buffer | E6315-4VIAL | -20 | 1 x 0.48 ml | ||
DNase I (RNase-free) | E6316-4VIAL | -20 | 1 x 0.24 ml | ||
Nuclease-free Water | E6317-4VIAL | -20 | 1 x 6 ml | ||
NEBNext RNase H | E6318-4VIAL | -20 | 1 x 0.192 ml |
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.This product and its use are subject to one or more issued and/or pending U.S. and foreign patent applications. Commercial use for the determination, prediction and prognosis of cancer, or response to therapy for cancer, in humans or animals may require a license. Please contact busdev@neb.com for more information.