SP6 RNA Polymerase is used for in vitro mRNA synthesis and is highly specific for the SP6 promoter.
Bacteriophage SP6 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the SP6 phage promoter. The 98.5 KD polymerase catalyzes in vitro RNA synthesis from a cloned DNA template under the SP6 promoter. RNA synthesized using the SP6 RNA Polymerase is suitable for many applications in research and biotechnology.
Reaction Conditions:
1X RNAPol Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP and DNA template containing the SP6 RNA Polymerase promoter. Incubate at 37°C.
1X RNAPol Reaction Buffer
Supplement with 0.5 mM ATP, 0.5 mM GTP, 0.5 mM UTPÂ and 0.5 mM CTPÂ
Incubate at 37°C
1X RNAPol Reaction Buffer
40 mM Tris-HCl
6 mM MgCl2
1 mM DTT
2 mM spermidine
(pH 7.9 @ 25°C)
50 mM Tris-HCl
100 mM NaCl
20 mM β-ME
1 mM EDTA
50% Glycerol
0.1% (w/v) Triton® X-100
pH 7.9 @ 25°C
Supplement reactions with fresh DTT if buffer is > 6 months old.
Increase yields by raising rNTP concentrations to 4 mM each and MgCl2 to 20 mM.
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