Poly(A) Polymerase catalyzes the template independent addition of AMP from ATP to the 3´ end of RNA
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
1X Poly(A) Polymerase Reaction Buffer
Supplement with 1 mM ATP
Incubate at 37°C
1X Poly(A) Polymerase Reaction Buffer
50 mM Tris-HCl
250 mM NaCl
10 mM MgCl2
(pH 8.1 @ 25°C)
20 mM Tris-HCl
300 mM NaCl
1 mM DTT
1 mM EDTA
50% Glycerol
0.1% (w/v) Triton® X-100
pH 7.5 @ 25°C
E. coli Poly (A) Polymerase (M0276)
Vortex tube gently before use.
Poly(A) Polymerase can be inactivated by adding EDTA at a final concentration of 10 mM to the reaction.
Poly (A) Polymerase is compatible with the M-MuLV reverse transcriptase buffer. If the intention is to carry out the polyadenylation step followed by reverse transcription, we recommend to set-up these reactions in two sequential steps. The polyadenylation reaction can be done first using the reverse transcriptase buffer. This can be followed by addition of the components necessary for reverse transcription.
We strongly recommend wearing gloves, using nuclease-free tubes and reagents, and thoroughly cleaning pipettes and bench surfaces to avoid RNase contamination.
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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