New England Biolabs Canada
Expand menu
Sign In or Sign UpMy NEB

We are transitioning to our new website at www.neb.com, which will go live on April 7th, 2025.

On April 2nd at 8:00 PM ET, www.neb.ca e-commerce functionality will be disabled for the website transition. If you need to place your order between April 2nd at 8:00 pm ET to April 6th, then please call 1-800-387-1095 or email orders.ca@neb.com.

The transition to the new website will not affect our shipping schedule. If you're a registered user, all your account details, including order history and shipping details will be safely transferred to the new site.

RNase Inhibitor, Murine

Catalog # Concentration Size List Price Quantity Your Price
M0314L 40000 units/ml 15000 units $489.00
$440.10
M0314S 40000 units/ml 3000 units $123.00
$110.70
Catalog # Size List Price Your Price
M0314L 15000 units $489.00
$440.10
M0314S 3000 units $123.00
$110.70
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

RNase Inhibitor, Murine, specifically inhibits RNases A, B and C.

  • Improved resistance to oxidation, compared to human/porcine RNase inhibitor
  • Ideal for reactions where low DTT concentrations are required (e.g., Real-time PCR)
  • Isolated from a recombinant source
  • Tested for the absence of DNases and RNases
  • Getting ready to scale up RNA synthesis? Download our new technical note “Scaling of High-Yield In vitro Transcription Reactions for Linear Increase of RNA Production” for a generalized set of recommendations for synthesizing high yields of RNA.
Featured Videos
View Video Library
  • Monarch_MiniprepProtocol_VideoThumb
    Avoiding RNase Contamination
RNase Inhibitor, Murine is a 50 kDa recombinant protein of murine origin. The inhibitor specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).

Recombinant murine RNase inhibitor does not contain the pair of cysteines identified in the human version that is very sensitive to oxidation, which causes inactivation of the inhibitor (1). As a result, RNase Inhibitor, Murine has significantly improved resistance to oxidation compared to the human/porcine RNase inhibitors, and is stable at low DTT concentrations (less than 1 mM). This makes it ideal for reactions where high concentration DTT is adverse to the reaction (eg. Real-time RT-PCR).
Product Source
An E. coli strain that carries the Ribonuclease Inhibitor gene from mouse.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  RNase Inhibitor, Murine M0314SVIAL -20 1 x 0.075 ml 40,000 units/ml
  RNase Inhibitor, Murine M0314LVIAL -20 1 x 0.375 ml 40,000 units/ml
Application Features
  • RT-PCR
  • cDNA synthesis
  • In vitro transcription/translation
  • Enzymatic RNA labeling reaction
  • Other applications where the integrity of RNA is important
RNase Inhibitor, Murine | New England Biolabs

RNase Inhibitor, Murine
recombinant

GMP-grade reagent also available. Learn more.

RNase Inhibitor, Murine, specifically inhibits RNases A, B and C.

  • Improved resistance to oxidation, compared to human/porcine RNase inhibitor
  • Ideal for reactions where low DTT concentrations are required (e.g., Real-time PCR)
  • Isolated from a recombinant source
  • Tested for the absence of DNases and RNases
  • Getting ready to scale up RNA synthesis? Download our new technical note “Scaling of High-Yield In vitro Transcription Reactions for Linear Increase of RNA Production” for a generalized set of recommendations for synthesizing high yields of RNA.

Featured Video

Catalog https://www.neb.com/en/# Concentration Size
M0314S 40,000 units/ml 3,000 units
M0314L 40,000 units/ml 15,000 units
Please enter a quantity for at least one size
Now available as a GMP-grade reagent. Learn more

Ineligible item added to cart

Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly. If you have any questions, please contact Customer Support at freezers@neb.com or 1-800-632-5227 x 8.

Continue

Choose your country

If you don&https://www.neb.com/en/#39;t see your country above, please visit our international site

Loading Spinner

Session Expired

You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.

Institution Changed

Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.

Sign in to your NEB account

To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.

Sign In



Notes
  • Since ribonucleases typically retain activity under denaturing conditions, care must be taken to avoid denaturing RNase Inhibitor molecules which have complexed with a ribonuclease. To prevent the release of active ribonuclease, temperatures greater than 50°C and high concentrations of urea or other denaturing agents should be avoided.
  • The recommended concentration of RNase Inhibitor in a reaction is 1 unit/μl. During assembly of a reaction, RNase Inhibitor should be added before other components that are a possible source of RNase contamination (i.e. enzymes, plasmid from a mini prep.)
References
  • Kim, B.M. et al. Protein Science. 8, 430-434.
Tech Tips
  • The inhibitor is a 50KD protein. It will be inactivated in conditions that will denature proteins.
  • We recommend using the inhibitor at 1U/ul in your reaction.
  • The inhibitor should be added in a reaction before other components that are a possible source of RNase contamination.
  • The inhibitor should be used below 50°C.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Other Products You May Be Interested In
 Top