The dsRNA Ladder is a set of 7 annealed double-stranded RNA molecules produced by in vitro transcription of 14 linear DNA templates. The ladder sizes are: 500, 300, 150, 80, 50, 30 and 21 base pairs. All have 2-base, 3´ extensions. The 80 base pair fragment is at double intensity to serve as a reference point.
This ladder is suitable for use as a size standard in dsRNA and RNAi analysis on both non-denaturing polyacrylamide and agarose gels.
Fragment | bp |
---|---|
1 | 500 |
2 | 300 |
3 | 150 |
4 | 80 |
5 | 50 |
6 | 30 |
7 | 21 |
10 mM Tris-HCl
1 mM EDTA
pH 8 @ 25°C
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