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Pyrophosphatase, Inorganic (E. coli)

Catalog # Concentration Size List Price Quantity Your Price
M0361L 100 units/ml 50 units $412.00
$370.80
M0361S 100 units/ml 10 units $102.00
$91.80
Catalog # Size List Price Your Price
M0361L 50 units $412.00
$370.80
M0361S 10 units $102.00
$91.80
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate.


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Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate:
Product Source
PPase is prepared from an E. coli strain containing an engineered His-tag E. coli inorganic pyrophophatase gene.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Pyrophosphatase, Inorganic (E. coli) M0361SVIAL -20 1 x 0.1 ml 100 units/ml
  Pyrophosphatase, Inorganic (E. coli) M0361LVIAL -20 1 x 0.5 ml 100 units/ml
Application Features
  • Increasing RNA yield in transcription reaction; enhancing DNA replication.

Properties & Usage

Unit Definition

One unit is the amount of enzyme that will generate 1 µmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25°C in 20 mM Tris-HCl, pH 8.0, 2 mM MgCl2 and 2 mM  PPi).

Storage Buffer

20 mM Tris-HCl
100 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 8 @ 25°C

Heat Inactivation

No

Notes
  • Use 1–3 units per ml in a high yield in vitro RNA synthesis reaction.
  • While a specific NEBuffer is not supplied, E. coli Inorganic Pyrophosphatase works well in any buffer containing Mg++ (1-10 mM). This enzyme is typically used in RNA IVT reactions, where the Mg++ concentration falls within this range.
Tech Tips
  • The enzyme works well in most reaction buffers containing Mg2+.
    It cannot be inactivated by heating at 70°C.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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