The NEBNext® Library Quant Kit for Illumina® has been updated to include six standards, enabling a broader standard curve. For users who are familiar with the previous four standards, numbering has changed (e.g., what was once Standards 1-4 is now Standards 2-5). Both the four-standard and six-standard protocols are supported; see the Manual for details.
Accurate quantitation of next-generation sequencing (NGS) libraries is essential for maximizing data output and quality from each sequencing run. For Illumina® sequencing specifically, accurate quantitation of libraries is critical to achieve optimal cluster densities, a requirement for optimal sequence output. qPCR is considered to be the most accurate and effective method of library quantitation, providing considerably higher consistency and reproducibility of quantitation. Further, amplification-based methods quantitate only those molecules that contain both adaptor sequences, thereby providing a more accurate estimate of the concentration of the library molecules that can be sequenced. The NEBNext® Library Quant Kit has been optimized to provide substantial performance and workflow improvements to qPCR-based library quantitation.
Additional dilution buffer can be purchased separately.
Additional standards can be purchased separately.
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
E7630S | -20 |
NEBNext® Library Quant Master Mix | E7640AVIAL | -20 | 1 x 1.5 ml | ||
NEBNext® Library Quant Primer Mix | E7639AVIAL | -20 | 1 x 0.1 ml | ||
NEBNext® Library Dilution Buffer | E7633AVIAL | -20 | 2 x 1 ml | 10 X | |
NEBNext® Library Quant DNA Standard 1 | E6886AVIAL | -20 | 1 x 0.024 ml | 100 pM | |
NEBNext® Library Quant DNA Standard 2 | E6887AVIAL | -20 | 1 x 0.024 ml | 10 pM | |
NEBNext® Library Quant DNA Standard 3 | E6888AVIAL | -20 | 1 x 0.024 ml | 1 pM | |
NEBNext® Library Quant DNA Standard 4 | E6889AVIAL | -20 | 1 x 0.024 ml | 0.1 pM | |
NEBNext® Library Quant DNA Standard 5 | E6890AVIAL | -20 | 1 x 0.024 ml | 0.01 pM | |
NEBNext® Library Quant DNA Standard 6 | E6891AVIAL | -20 | 1 x 0.024 ml | 0.001 pM | |
ROX (High) | E7641AVIAL | -20 | 1 x 0.02 ml | ||
ROX (Low) | E7638AVIAL | -20 | 1 x 0.02 ml |
E7630L | -20 |
NEBNext® Library Quant Master Mix | E7640AAVIAL | -20 | 1 x 7.5 ml | ||
NEBNext® Library Quant Primer Mix | E7639AAVIAL | -20 | 1 x 0.5 ml | ||
NEBNext® Library Dilution Buffer | E7633AAVIAL | -20 | 2 x 7.5 ml | 10 X | |
NEBNext® Library Quant DNA Standard 1 | E6886AAVIAL | -20 | 1 x 0.12 ml | 100 pM | |
NEBNext® Library Quant DNA Standard 2 | E6887AAVIAL | -20 | 1 x 0.12 ml | 10 pM | |
NEBNext® Library Quant DNA Standard 3 | E6888AAVIAL | -20 | 1 x 0.12 ml | 1 pM | |
NEBNext® Library Quant DNA Standard 4 | E6889AAVIAL | -20 | 1 x 0.12 ml | 0.1 pM | |
NEBNext® Library Quant DNA Standard 5 | E6890AAVIAL | -20 | 1 x 0.12 ml | 0.01 pM | |
NEBNext® Library Quant DNA Standard 6 | E6891AAVIAL | -20 | 1 x 0.12 ml | 0.001 pM | |
ROX (High) | E7641AAVIAL | -20 | 1 x 0.1 ml | ||
ROX (Low) | E7638AAVIAL | -20 | 1 x 0.1 ml |
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the NEBNext® Library Quant Kit for Illumina® for Research Use Only (RUO). Commercial use of this product may require a license from New England Biolabs, Inc. For additional information or to inquire about commercial use, please contact busdev@neb.com.