NEBNext® Library Quant DNA Standards

Catalog #	Concentration	Size	List Price	Quantity	Your Price
E7642S		500 reactions	$289.00		$260.10

Catalog #	Size	List Price	Your Price
E7642S	500 reactions	$289.00	$260.10

For use with the NEBNext Library Quant Master Mix included in the NEBNext Libarary Quant Kit for Illumina, this product includes six tubes of standards (100 pM to 0.001 pM) providing extra volume for users who require it. Protocols included in the (NEB #E7630) product manual detail the process for setting up standard curves using either four or six standards, building flexibility into the workflow.

The NEBNext Library Quant Kit for Illumina offers a streamlined protocol and reduced lot-to-lot variability as compared to similar library quantification kits.

Typical results from the NEBNext Library Quant Kit on a Bio-Rad CFX96 Touch (A) and an Applied Biosystems 7500 Fast real-time qPCR instrument (B). Amplification curves are shown on the left and resulting standard curves on the right. All default settings were used on both platforms. ABI 7500 Fast assay included 1X ROX (Low Concentration) and the ROX normalization.

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

E7642S -20

NEBNext^® Library Quant DNA Standard 1	E6886AAVIAL	-20	1 x 0.12 ml	100 pM
NEBNext^® Library Quant DNA Standard 2	E6887AAVIAL	-20	1 x 0.12 ml	10 pM
NEBNext^® Library Quant DNA Standard 3	E6888AAVIAL	-20	1 x 0.12 ml	1 pM
NEBNext^® Library Quant DNA Standard 4	E6889AAVIAL	-20	1 x 0.12 ml	0.1 pM
NEBNext^® Library Quant DNA Standard 5	E6890AAVIAL	-20	1 x 0.12 ml	0.01 pM
NEBNext^® Library Quant DNA Standard 6	E6891AAVIAL	-20	1 x 0.12 ml	0.001 pM

Application Features

Additional volume of the same standards included in the NEBNext Library Quant Kit for Illumina
qPCR-based library quant based on either four or six standards

FAQs

Citations

See more details on Bioz

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

E7642S_v2

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.