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NEB® Turbo Competent E. coli (High Efficiency)

Catalog # Concentration Size List Price Quantity Your Price
C2984H 20 x 0.05 ml $436.00
$392.40
C2984I 6 x 0.2 ml $331.00
$297.90
Catalog # Size List Price Your Price
C2984H 20 x 0.05 ml $436.00
$392.40
C2984I 6 x 0.2 ml $331.00
$297.90
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

E. coli cells featuring fast colony growth (6.5 hours)

  • Tight expression control (lacIq)
  • Isolate DNA after only 4 hours of growth
  • 5-minute transformation protocol with AmpR plasmids
  • No dry ice surcharge on competent cell shipments
  • Outgrowth medium included
  • Free of animal products
Featured Videos
View Video Library
  • Monarch_MiniprepProtocol_VideoThumb
    Tips for Successful Transformations with NEB® Competent Cells
  • Monarch_MiniprepProtocol_VideoThumb
    How to Perform a Transformation with NEB® Competent Cells
  • Monarch_MiniprepProtocol_VideoThumb
    The Mechanism of Transformation with Competent Cells
  • Monarch_MiniprepProtocol_VideoThumb
    Overview of PCR Cloning
  • Monarch_MiniprepProtocol_VideoThumb
    Traditional Cloning Workflow
  • Monarch_MiniprepProtocol_VideoThumb
    Fast transformation plating with glass beads
Chemically competent E. coli cells suitable for high efficiency transformation and rapid colony growth.
Highlights
  • Tight control of expression by laclq allows potentially toxic genes to be cloned
  • Highest growth rate on agar plates - visible colonies 6.5 hours after transformation
  • Activity nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
  • Resistance to phage T1 (fhuA2)
  • Suitable for blue/white screening by α-complementation of the β-galactosidase gene
  • Suitable for 5 minute transformation protocol with AmpR plasmids
  • EcoKr-m-, McrBC-
  • K12 Strain
  • Isolate DNA after 4 hours of growth from a single overnight colony
  • Free of animal products
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl
  NEB® Turbo Competent E. coli (High Efficiency) C2984HVIAL -80 20 x 0.05 ml
  SOC Outgrowth Medium B9020SVIAL 4 1 x 25 ml
  pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl
  NEB® Turbo Competent E. coli (High Efficiency) C2984IVIAL -80 6 x 0.2 ml
  SOC Outgrowth Medium B9020SVIAL 4 1 x 25 ml
Features
  • Fastest growth – colonies visible after 6.5 hours
  • Plasmid preparation after 4 hours
  • Tight control/expression of toxic genes (F´lacIq)
Application Features

DNA Effects on Transformation Efficiency and Colony Output: The optimal amount of DNA to use in a transformation reaction is lower than commonly recognized. Using clean, supercoiled pUC19, the efficiency of transformation is highest in the 100 pg-1 ng range. However, the total colonies which can be obtained from a single transformation reaction increase up to about 100 ng.
DNA Effects on Transformation Efficiency and Colony Output: DNA Effects on Transformation Efficiency and Colony Output:
The optimal amount of DNA to use in a transformation reaction is lower than commonly recognized. Using clean, supercoiled pUC19, the efficiency of transformation is highest in the 100 pg-1 ng range. However, the total colonies which can be obtained from a single transformation reaction increase up to about 100 ng.

Growth curve of NEB Turbo vs DH5α at 37°C in shaking flask:Growth curve of NEB Turbo vs DH5α at 37°C in shaking flask:
NEB Turbo and DH5α frozen stock (same cell density) were inoculated 1:100 into 80 ml of liquid medium (10g tryptone, 5g yeast extract, 10g NaCl, 1g MgCl2 and 1g Dextrose per liter, pH7.2) in 1 liter flask, respectively. Cells were grown at 37°C with shaking at 250 rpm. O.D.600 was measured every 30 minutes for up to 16 hours.
Growth curve of NEB Turbo vs DH5α at 37°C in fermentor: Growth curve of NEB Turbo vs DH5α at 37°C in fermentor
NEB Turbo and DH5α cultures grown overnight were inoculated 1:50 into 1 liter of LB (10g tryptone, 5g yeast extract, 10g NaCl per liter, pH7.0) in 1 liter fermentor, respectively. Cells were grown at 37°C. O.D.600 was measured every 30 minutes for up to 9 hours.
NEB Turbo cell growth and DNA yield: NEB Turbo cell growth and DNA yield:
pUC19 was transformed into NEB Turbo competent E.coli. Six colonies grown overnight (colony size was about 2.4 mm in diameter) were inoculated into 30 ml of LB/Amp, mixed thoroughly and 5 ml was dispensed into each 25 ml test tube (equal to 1 colony in 5 ml of LB/Amp). The cells were grown at 37°C with shaking at 250 rpm. One test tube was placed on ice after 3, 4, 5, 6, 7 and 16 hours of growth, respectively and the O.D.600 was measured. Mini-prep was performed by spinning down 1.5 ml of culture 3 times (total 4.5 ml) and finally eluting the DNA into 50 μl of EB buffer.
Comparison of DNA yield after 4 hours of growth: Comparison of DNA yield after 4 hours of growth:
pUC19 was transformed into NEB Turbo competent E.coli, Invitrogen Mach1 and DH5α, respectively. One overnight grown colony (colony size for NEB Turbo was about 2.4 mm in diameter, 1.8 mm for Mach1 and 1.2 mm for DH5α) was inoculated into 5 ml of LB/Amp in a 25ml test tube and cells were grown at 37°C with shaking at 250 rpm. After 4 hours of growth, cells were measured O.D.600 and mini-prep was performed by spinning down 1.5 ml of culture 3 times (total 4.5 ml) and finally eluting the DNA into 50 μl of EB buffer. O.D.600 for NEB Turbo was 3.08, 2.36 for Mach1 and 0.75 for DH5α.

Effect of heat shock time on NEB Turbo competent E.coli transformation efficiency:Effect of heat shock time on NEB Turbo competent E.coli transformation efficiency:
50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except heat shock time varied from 0 to 80 seconds.
Effect of DNA incubation time on NEB Turbo competent E.coli transformation efficiency:Effect of DNA incubation time on NEB Turbo competent E.coli transformation efficiency:
50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except DNA incubation time varied from 0 to 40 minutes.
Effect of outgrowth medium on transformation efficiency: Effect of outgrowth medium on transformation efficiency:
50 μl of NEB Turbo competent E.coli was transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol with the exception of varying the outgrowth medium. NEB SOC outgrowth medium delivers the highest transformation efficiency.

Properties & Usage

Antibiotic for Plasmid Selection

Antibiotics for Plasmid Selection Working Concentration
Ampicillin 100 µg/ml
Carbenicillin 100 µg/ml
Chloramphenicol 33 µg/ml
Kanamycin 30 µg/ml
Streptomycin 25 µg/ml
Tetracycline 15 µg/ml

Shipping Notes

  • Ships on dry ice

Antibiotic Resistance

  • nit


Companion Products
Materials Sold Separately
Notes
  • CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
  • STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.
FAQs
Quality Control Assay
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Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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