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dam-/dcm- Competent E. coli

Catalog # Concentration Size List Price Quantity Your Price
C2925H 20 x 0.05 ml $405.00
$364.50
C2925I 6 x 0.2 ml $310.00
$279.00
Catalog # Size List Price Your Price
C2925H 20 x 0.05 ml $405.00
$364.50
C2925I 6 x 0.2 ml $310.00
$279.00
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Methyltransferase deficient E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation

  • No dry ice surcharge on competent cell shipments
  • Outgrowth medium included
  • Free of animal products
Featured Videos
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  • Monarch_MiniprepProtocol_VideoThumb
    Tips for Successful Transformations with NEB® Competent Cells
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    Fast transformation plating with glass beads
Methyltransferase deficient chemically competent E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation. Note that dam- strains are not recommended as a host for primary cloning/ligation. The dam mutation can result in an increased mutation rate in the cell and a reduction in the transformation efficiency. DNA should be maintained in a dam+ strain unless there is a specific need for DNA free of Dam or Dcm methylation.
Highlights
  • Grow plasmids free of Dam and Dcm methylation
  • Free of animal products
  • Phage T1 resistant (fhuA31)
  • Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
  • K12 Strain
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl
  SOC Outgrowth Medium B9020SVIAL 4 1 x 25 ml
  dam–/dcm– Competent E. coli C2925HVIAL -80 20 x 0.05 ml
  pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl
  SOC Outgrowth Medium B9020SVIAL 4 1 x 25 ml
  dam–/dcm– Competent E. coli C2925IVIAL -80 6 x 0.2 ml
Features
  • Dam/Dcm methyltransferase free plasmid growth
Application Features

Effect of heat shock time on dam-/dcm- competent E. coli transformation efficiency:Effect of heat shock time on dam-/dcm- competent E. coli transformation efficiency:
50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except heat shock time varied from 0 to 80 seconds.
Effect of DNA incubation time on dam-/dcm- competent E. coli transformation efficiency: Effect of DNA incubation time on dam-/dcm- competent E. coli transformation efficiency
50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except DNA incubation time varied from 0 to 40 minutes.
Effect of outgrowth medium on transformation efficiency: Effect of outgrowth medium on transformation efficiency:
50 μl of -/dcm-competentE.coli was transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol with the exception of varying the outgrowth medium. NEB SOC outgrowth medium delivers the highest transformation efficiency.

Properties & Usage

Antibiotic for Plasmid Selection

Antibiotics for Plasmid Selection Working Concentration
Ampicillin 100 µg/ml
Carbenicillin 100 µg/ml
Kanamycin 30 µg/ml
Tetracycline 15 µg/ml

Shipping Notes

  • Ships on dry ice

Antibiotic Resistance

  • str
  • nit
  • cam


Companion Products
Materials Sold Separately
Notes
  • CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
  • STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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