NEB® 5-alpha F'Iq Competent E. coli (High Efficiency)

Catalog #	Concentration	Size	List Price	Quantity	Your Price
C2992H		20 x 0.05 ml	$326.00		$293.40
C2992I		6 x 0.2 ml	$255.00		$229.50

Catalog #	Size	List Price	Your Price
C2992H	20 x 0.05 ml	$326.00	$293.40
C2992I	6 x 0.2 ml	$255.00	$229.50

Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications.

Highlights

DH5α™ derivative
Free of animal products
Efficient transformation of unmethylated DNA derived from PCR, cDNA and many other sources (hsdR)
Tight control of expression by lacl^q allows potentially toxic genes to be cloned
Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
Resistance to phage T1 (fhuA2)
Suitable for blue/white screening by α-complementation of the β-galactosidase gene
F' allows cells to be infected with bacteriophage M13 for ssDNA production
Reduced recombination of cloned DNA (recA1)
K12 Strain

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

C2992H -80

SOC Outgrowth Medium	B9020SVIAL	4	1 x 25 ml
pUC19 Vector	N3041AVIAL	-20	1 x 0.025 ml	50 pg/µl
NEB^® 5-alpha F'I^q Competent E. coli (High Efficiency)	C2992HVIAL	-80	20 x 0.05 ml

C2992I -80

SOC Outgrowth Medium	B9020SVIAL	4	1 x 25 ml
pUC19 Vector	N3041AVIAL	-20	1 x 0.025 ml	50 pg/µl
NEB^® 5-alpha F'I^q Competent E. coli (High Efficiency)	C2992IVIAL	-80	6 x 0.2 ml

Features

Toxic gene cloning
F´ strain with extremely high transformation efficiency

Application Features

DNA Effects on Transformation Efficiency and Colony Output — The optimal amount of DNA to use in a transformation reaction is lower than commonly recognized. Using clean, supercoiled pUC19, the efficiency of transformation is highest in the 100 pg-1 ng range. However, the total colonies which can be obtained from a single transformation reaction increase up to about 100 ng.

Effect of heat shock time on NEB 5-alpha F´Iq competent E.coli transformation efficiency: — 50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except heat shock time varied from 0 to 80 seconds.

Effect of DNA incubation time on NEB 5-alpha F´Iq competent E.coli transformation efficiency: — 50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except DNA incubation time varied from 0 to 40 minutes.

Effect of outgrowth medium on transformation efficiency: — 50 μl of NEB 5-alpha F´Iq competent E.coli was transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol with the exception of varying the outgrowth medium. NEB SOC outgrowth medium delivers the highest transformation efficiency.

Properties & Usage

Antibiotic for Plasmid Selection

Antibiotics for Plasmid Selection	Working Concentration
Ampicillin	100 Âµg/ml
Carbenicillin	100 Âµg/ml
Chloramphenicol	33 Âµg/ml
Kanamycin	30 Âµg/ml
Streptomycin	25 Âµg/ml

Shipping Notes

Ships on dry ice

Antibiotic Resistance

tetracycline

Companion Products

Materials Sold Separately

SOC Outgrowth Medium

Notes

CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.

Protocols

Usage Guidelines

Selection Charts

Web Tools

FAQs

Citations

See more details on Bioz

Additional Citations

Firnberg E, Ostermeier M (2013) The genetic code constrains yet facilitates Darwinian evolution Nucleic Acids Res 41(15), 7420-8.PubMedID: 23754851, DOI: 10.1093/nar/gkt536

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

C2992H_I_v1

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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