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NEB® 10-beta Competent E. coli (High Efficiency)

Catalog # Concentration Size List Price Quantity Your Price
C3019H 20 x 0.05 ml $376.00
$338.40
C3019I 6 x 0.2 ml $289.00
$260.10
C3019P 1 96-well pl $854.00
$768.60
Catalog # Size List Price Your Price
C3019H 20 x 0.05 ml $376.00
$338.40
C3019I 6 x 0.2 ml $289.00
$260.10
C3019P 1 96-well pl $854.00
$768.60
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

NEB 10-beta Competent E. coli is a derivative of the popular DH10B. It is T1 phage resistant and endonuclease I (endA1) deficient for high- quality plasmid preparations.

  • High efficiency strain ideal for cloning large plasmids and BACs
  • Available in single-use vials, 200 μl vials, 96-well plate and electrocompetent format
  • No dry ice surcharge on competent cell shipments
  • Outgrowth medium included
  • Free of animal products
Featured Videos
View Video Library
  • Monarch_MiniprepProtocol_VideoThumb
    Tips for Successful Transformations with NEB® Competent Cells
  • Monarch_MiniprepProtocol_VideoThumb
    How to Perform a Transformation with NEB® Competent Cells
  • Monarch_MiniprepProtocol_VideoThumb
    The Mechanism of Transformation with Competent Cells
  • Monarch_MiniprepProtocol_VideoThumb
    Overview of PCR Cloning
  • Monarch_MiniprepProtocol_VideoThumb
    Traditional Cloning Workflow
  • Monarch_MiniprepProtocol_VideoThumb
    NEBUILDER HIFI DNA ASSEMBLY® Removal of 3´ end Mismatches
  • Monarch_MiniprepProtocol_VideoThumb
    Fast transformation plating with glass beads

Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications.

Highlights
  • DH10B™ derivative
  • Efficient transformation of methylated DNA derived from eukaryotic sources or unmethylated DNA derived from PCR, cDNA and many other sources [mcrAΔ(mrr-hsdRMS-mcrBC)]
  • Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
  • Resistance to phage T1 (fhuA)
  • Suitable for blue/white screening without IPTG by a-complementation of the b-galactosidase gene
  • Reduced recombination of cloned DNA (recA1)
  • Clone large plasmids and BACs
  • Free of animal products
  • K12 Strain
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  NEB® 10-beta/Stable Outgrowth Medium B9035SVIAL 4 1 x 25 ml
  pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl
  NEB® 10-beta Competent E. coli (High Efficiency) C3019HVIAL -80 20 x 0.05 ml
  NEB® 10-beta/Stable Outgrowth Medium B9035SVIAL 4 1 x 25 ml
  pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl
  NEB® 10-beta Competent E. coli (High Efficiency) C3019IVIAL -80 6 x 0.2 ml
  pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl
  NEB® 10-beta/Stable Outgrowth Medium B9035SVIAL 4 1 x 25 ml
  NEB® 10-beta Competent E. coli (High Efficiency) C3019PVIAL -80 1 x20 μl/well
Features
  • Large plasmid and BAC cloning
  • DH10B™ derivative
Application Features

Effect of plasmid size on transformation efficiency Effect of Plasmid Size on Transformation Efficiency:
NEB 10-beta chemically competent cells (C3019H) are more efficiently transformed with large plasmids than NEB 5-alpha cells (C2987H). The difference in TE between the two cell lines increases with the size of the plasmid being transformed.

Effect of heat shock time on NEB 10-beta competent E. coli transformation efficiency Effect of heat shock time on NEB 10-beta competent E. coli transformation efficiency:   50 μl of competent cells (C3019H) were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except heat shock time varied from 0 to 80 seconds.

Effect of DNA incubation time on NEB 10-beta competent E. coli transformation efficiency Effect of DNA incubation time on NEB 10-beta competent E. coli transformation efficiency:
50 μl of competent cells (C3019H) were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except DNA incubation time varied from 0 to 40 minutes.

Effect of outgrowth medium on transformation efficiency Outgrowth
50 μl of NEB 10-beta competent E. coli (C3019H) was transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol with the exception of varying the outgrowth medium. NEB 10-beta/Stable Outgrowth Medium delivers the highest transformation efficiency.

Benefit from the high transformation efficiencies of NEB 10-beta (C3019H/I) Efficiency
Transformation efficiencies were compared using manufacturers’ recommended protocols. Values shown are the average of triplicate experiments.

Properties & Usage

Antibiotic for Plasmid Selection

Antibiotics for Plasmid Selection Working Concentration
Ampicillin 100 µg/ml
Carbenicillin 100 µg/ml
Chloramphenicol 33 µg/ml
Kanamycin 30 µg/ml
Tetracycline 15 µg/ml

Shipping Notes

  • Ships on dry ice

Antibiotic Resistance

  • str


Materials Sold Separately
Notes
  • STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.
  • CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.ONESHOT® and MAX EFFICIENCY® are registered trademarks of Thermo Fisher Scientific.
DH10B™ is a trademarks of Thermo Fisher Scientific.

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