Bst 2.0 WarmStart® DNA Polymerase (Glycerol-free)

Catalog #	Concentration	Size	List Price	Quantity	Your Price
M0402L	120000 units/ml	8000 units	$625.00		$562.50

Catalog #	Size	List Price	Your Price
M0402L	8000 units	$625.00	$562.50

Bst 2.0 WarmStart DNA Polymerase (Glycerol-free) is formulated without glycerol to support lyophilization, incorporation into microfluidic devices, and enable quick adoption into automation workflows.

Bst 2.0 WarmStart DNA Polymerase is an in silico designed homolog of Bacillus stearothermophilus DNA Polymerase I, Large Fragment (Bst DNA Polymerase, Large Fragment) with a reversibly-bound aptamer, which inhibits polymerase activity at temperatures below 45°C. At temperatures above 45°C, the aptamer rapidly releases from the enzyme with no special activation step needed to activate the polymerase. Bst 2.0 WarmStart DNA Polymerase contains 5´→3´ DNA polymerase activity and strong strand-displacement activity but lacks 5´→3´ exonuclease activity. Bst 2.0 WarmStart DNA Polymerase displays improved amplification speed, yield, salt tolerance, and thermostability compared to wild-type Bst DNA Polymerase, Large Fragment.

This product is a core component of the LyoPrime WarmStart^® Fluorescent LAMP/RT-LAMP Mix (with UDG), a lyophilized LAMP mix offering ambient/room temperature storage and shipping and streamlined assay development. To learn about the lyophilization process, please visit www.neb.com/lyoprime.

M0402 DNA Targets — LAMP (DNA targets) experiments were performed with NEB #M0538: Bst 2.0 WarmStart DNA Polymerase or NEB #M0402: Bst 2.0 WarmStart DNA Polymerase (Glycerol-free). Reactions containing 1X LAMP primers and 0.5X LAMP Fluorescent dye were set up in quadruplicate over three logs of total Jurkat DNA (10 ng to 0.1 ng) in 96-well, 25 µl reactions. Control reactions without template (NTC) were also evaluated. Reactions were incubated at 65°C for 40 minutes and fluorescence was monitored every 15 seconds in the SYBR/FAM channel of a real-time thermocycler (Bio-Rad^® CFX96). Each dot represents the time at which the fluorescence signal for a single reaction crosses the instrument-defined threshold. All four replicates were detected at each template input unless otherwise indicated (note that dots frequently overlap given similar detection time for the replicates). Overall, similar performance was observed for both glycerol-containing (*NEB #M0538*) and glycerol-free (NEB #M0402) enzymes at each template input. No amplification was observed in any of the no template control reactions.

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

M0402L -80

	Bst 2.0 WarmStart^® DNA Polymerase (Glycerol-free)	M0402LVIAL	-80	1 x 0.067 ml	120,000 units/ml
	10X Isothermal Amplification Buffer (Lyo-compatible)	B1714SVIAL	-20	2 x 1.25 ml	10 X

An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Protocols

Typical LAMP Protocol (NEB #M0402)

Web Tools

NEB LAMP Primer Design Tool

FAQs

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

M0402L_v1

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

Bst 2.0 WarmStart^® DNA Polymerase (Glycerol-free)

Legal And Disclaimer

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.