WarmStart® RTx Reverse Transcriptase (Glycerol-free)

Catalog #	Concentration	Size	List Price	Quantity	Your Price
M0439L	75000 units/ml	2000 units	$539.00		$485.10

Catalog #	Size	List Price	Your Price
M0439L	2000 units	$539.00	$485.10

WarmStart RTx Reverse Transcriptase (Glycerol-free) is formulated without glycerol to support lyophilization, incorporation into microfluidic devices, and enable quick adoption into automation workflows.

WarmStart RTx Reverse Transcriptase is a unique in silico-designed RNA-directed DNA polymerase coupled with a reversibly-bound aptamer that inhibits RTx activity below 40°C. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA (cDNA synthesis) or single-stranded DNA as a template. RTx is a robust enzyme for RNA detection in amplification reactions and is particularly well suited for use in LAMP (Loop-mediated Isothermal Amplification). The WarmStart property enables high throughput applications, room temperature setup, and increases the consistency and specificity of amplification reactions. RTx contains intact RNase H activity.

This product is a core component of the LyoPrime WarmStart® Fluorescent LAMP/RT-LAMP Mix (with UDG), a lyophilized LAMP mix offering ambient/room temperature storage and shipping and streamlined assay development. To learn about the lyophilization process, please visit www.neb.com/lyoprime.

M0439 Targets — RT-LAMP (RNA targets) experiments were performed with NEB #M0380: WarmStart RTx Reverse Transcriptase and NEB #M0538: Bst 2.0 WarmStart^® DNA Polymerase, or NEB #M0439: WarmStart RTx Reverse Transcriptase (Glycerol-free) and NEB #M0402: Bst 2.0 WarmStart DNA Polymerase (Glycerol-free). Reactions containing 1X LAMP primers and 0.5X LAMP Fluorescent dye were set up in quadruplicate over three logs of total Jurkat RNA (10 ng to 0.1 ng) in 96-well, 25 µl reactions. Control reactions without template (NTC) were also evaluated. Reactions were incubated at 65°C for 40 minutes and fluorescence was monitored every 15 seconds in the SYBR/FAM channel of a real-time thermocycler (Bio-Rad^® CFX96). Each dot represents the time at which the fluorescence signal for a single reaction crosses the instrument-defined threshold. All four replicates were detected at each template input unless otherwise indicated (note that dots frequently overlap given similar detection time for the replicates). Overall, similar performance was observed for both glycerol-containing (NEB #M0538 and *NEB #M0380*) and glycerol-free (*NEB #M0402*: and NEB #M0439) enzymes at each template input. No amplification was observed in any of the no template control reactions.

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

M0439L -80

	WarmStart^® RTx Reverse Transcriptase (Glycerol-free)	M0439LVIAL	-80	1 x 0.027 ml	75,000 units/ml
	10X Isothermal Amplification Buffer (Lyo-compatible)	B1714SVIAL	-20	1 x 1.25 ml	10 X

An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Protocols

Web Tools

NEB LAMP Primer Design Tool

FAQs

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

M0439L_v1

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

WarmStart^® RTx Reverse Transcriptase (Glycerol-free)

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