Thermostable Endonuclease Q

Catalog #	Concentration	Size	List Price	Quantity	Your Price
M0701S	1000 units/ml	50 units	$128.00		$115.20

Catalog #	Size	List Price	Your Price
M0701S	50 units	$128.00	$115.20

Thermostable Endonuclease Q (EndoQ) is an archaeal DNA endonuclease that cleaves the phosphate backbone of a DNA substrate 5′ to the position of the modified nucleobases of deoxyxanthosine (dX), deoxyinosine (dI), deoxyuracil (dU), and an abasic (AP) site. The result is a 3′-OH and a 5′-phosphate. Thermostable Endonuclease Q generally prefers single-stranded DNA over double-stranded DNA, and exhibits higher activity on dU over dI and dX.

Product Source: An E. coli strain that carries a cloned archaeal Endonuclease Q gene with a C-terminal his-tag.

Relative Activity of Thermostable Endonuclease Q — Thermostable Endonuclease Q has different specificities for modified nucleotides in ssDNA and dsDNA. Thermostable Endonuclease Q (#M0701) activity was tested on 1 pmol of ssDNA or dsDNA substrates with fluorescently-labeled oligonucleotides containing modified nucleotides of interest (red) for 1 hour in 1x NEBuffer r2.1 at 65°C. Cleaved products were detected by capillary electrophoresis. Thermostable Endonuclease Q prefers ssDNA, and exhibits higher activity on dU over dI and dX.

Figure 2: Temperature Profile of Thermostable Endonuclease Q

Thermostable Endonuclease Q is active over a wide range of temperatures. Thermostable Endonuclease Q (#M0701, 0.2 U) activity was tested on 1 pmol of a fluorescently-labeled single-stranded oligonucleotide containing a single uracil for 1 hour in 1x NEBuffer r2.1 at various temperatures. Cleaved products were detected by capillary electrophoresis. Thermostable Endonuclease Q is active over a wide range of temperatures (37°C – 90°C), but displays optimal activity between 65°C and 85°C.

Thermostable Endonuclease Q active in some DNA:RNA — Thermostable Endonuclease Q is active on some DNA:RNA contexts. Thermostable Endonuclease Q (#M0701, 0.2 U) activity was tested on 1 pmol of substrate containing a single deoxyuracil (dU) or ribouracil (rU) within a DNA oligonucleotide (gray) or RNA oligonucleotide (blue) for 1 hour in 1x ThermoPol^®Reaction Buffer. Activity was compared with Thermostable USER^® III Enzyme (#M5509) for comparison. Cleaved products were detected by capillary electrophoresis. Thermostable Endonuclease Q can cleave at dU and rU located within DNA oligonucleotide strands regardless of presence or type (DNA or RNA) of complement.

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

M0701S -20

	Thermostable Endonuclease Q	M0701SVIAL	-20	1 x 0.05 ml	1,000 units/ml
	NEBuffer™ r2.1	B6002SVIAL	-20	1 x 1.25 ml	10 X

An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Materials Sold Separately

NEBuffer™ r2.1

Protocols

Protocol for Thermostable Endonuclease Q (NEB #M0701)

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Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

M0701S_v1

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

M0701S_v1_10260082

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