The Monarch Mag Viral DNA/RNA Extraction Kit enables reliable, high-throughput purification of viral nucleic acid using a magnetic bead-based protocol.
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Properties | |
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Purification format | Magnetic bead |
Processing format | Manual or automated |
Sample purification (representative examples) | Viral DNA and RNA* from respiratory viruses (enveloped and non-enveloped, dsDNA and ssRNA) |
Sample sources | Saliva, respiratory swab in viral transport media (VTM)** |
Sample input volume | Up to 200 μl** |
Carrier supplier | Poly A carrier RNA*** |
Binding capacity | Up to 3 μg |
Elution volume | 33–100 μl |
Tested automation platforms | KingFisher Flex; Agilent Bravo and MGISP liquid handlers |
Compatible downstream applications | qPCR, RT-qPCR, ddPCR, library prep for NGS |
* Viral DNA and RNA are purified in parallel. Preparation of DNA-free RNA or RNA-free DNA requires further treatment with the appropriate nuclease (not supplied).
** The sample input volume may be scalable to accommodate larger sample volumes. Further workflow optimization may be required.
*** Use of carrier RNA is recommended for recovery of low amounts of viral nucleic acid. Carrier RNA should be omitted if the downstream application utilizes poly(A) RNA enrichment; however, viral nucleic acid recovery may be reduced.
Silica-coated Monarch Mag Beads M1 are utilized for highly sensitive nucleic acid capture from samples containing few target molecules. Silanol groups on the bead shell provide an optimal binding surface for nucleic acids, and the uniform, submicron bead size offers a high surface area with abundant binding sites. Beads are supplied as a monodispersed suspension. The superparamagnetic properties of the bead core result in a fast magnetic response, contributing to ease of handling during use and compatibility with automation
An optimized viral nucleic acid extraction procedure employs a sample lysis step followed by a simple bind-wash-elute process. Samples are treated with Proteinase K and then mixed with a lysis buffer/bead mixture containing lysis buffer, carrier RNA, isopropanol, and magnetic beads, which promote binding of viral nucleic acid onto the silica-coated beads. Carrier RNA is utilized as a co-precipitant in the workflow to enhance the recovery of low amounts of viral nucleic acid. After binding of viral DNA and RNA to the magnetic beads, the beads are washed to remove contaminants, and nucleic acid is eluted in nuclease-free water. Purified viral nucleic acid is suitable for downstream applications, including qPCR/RT-qPCR, ddPCR, and library prep for Next Generation Sequencing (NGS).
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
T4010S | 25 |
Monarch® Mag Beads M1 | T4005-21 | 25 | 1 x 2.4 ml | ||
Monarch® Carrier RNA | T4006-21 | 25 | 1 x 125 µg | ||
Monarch StabiLyse™ DNA/RNA Buffer | T2111-1 | 25 | 1 x 24 ml | ||
Monarch® Buffer BX | T2041-1 | 25 | 1 x 19 ml | ||
Nuclease-free Water | B1500-2 | 25 | 1 x 25 ml | ||
Proteinase K, Molecular Biology Grade | P8200AAVIAL | -20 | 1 x 0.55 ml | 20 mg/ml |
T4010L | 25 |
Monarch® Mag Beads M1 | T4005-1 | 25 | 1 x 14 ml | ||
Monarch® Carrier RNA | T4006-1 | 25 | 1 x 750 µg | ||
Monarch StabiLyse™ DNA/RNA Buffer | T2111-3 | 25 | 1 x 145 ml | ||
Monarch® Buffer BX | T2041-3 | 25 | 1 x 122 ml | ||
Nuclease-free Water | B1500-3 | 25 | 1 x 122 ml | ||
Proteinase K, Molecular Biology Grade | P8200SVIAL | -20 | 3 x 1 ml | 20 mg/ml | |
Proteinase K, Molecular Biology Grade | P8200AAVIAL | -20 | 1 x 0.55 ml | 20 mg/ml |
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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