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λ DNA-Mono Cut Mix

Catalog # Concentration Size List Price Quantity Your Price
N3019S 500 µg/ml 100 gel lanes $121.00
$108.90
Catalog # Size List Price Your Price
N3019S 100 gel lanes $121.00
$108.90
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • The Mono Cut Mix is a mixture of intact lambda DNA and lambda DNA separately digested with ApaI, KpnI, XbaI and XhoI.
  • The fragments have been filled in with DNA Polymerase I Large (Klenow) Fragment to prevent re-annealing.
  • The approximate mass of DNA in each of the bands is provided (assuming a 0.5 μg load) for approximating the mass of DNA in comparably intense samples of similar size.
  • For pulsed-field gel electrophoresis (PFGE)
  • May also be used for standard electrophoresis
  • Size range: 1,503 bp to 48,502 bp
  • Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
Featured Videos
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  • Monarch_MiniprepProtocol_VideoThumb
    How to Prepare and Load a Standard DNA Ladder
  • Monarch_MiniprepProtocol_VideoThumb
    What is Pulsed Field Gel Electrophoresis?
  • Recommended gel and migration conditions: PFGE on CHEF system, 1% 0.5X TBE agarose, 6V/cm, 15C, 0.5 to 1.5 seconds for 20 hours.

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  λ DNA-Mono Cut Mix N3019SVIAL -20 1 x 0.1 ml 500 µg/ml
  Gel Loading Dye, Purple (6X), no SDS B7025SVIAL 25 1 x 1 ml 6 X

Properties & Usage

Bases

Fragment Mass (ng) bp
1 100 48502
2 79 38416
3 69 33498
4 62 29946
5 51 24508
6 49 23994
7 35 17053
8 31 15004
9 21 10086
10 3 1503

Effective Size Range

1,503bp to 48,502bp

Storage Buffer

10 mM Tris-HCl
1 mM EDTA
pH 8 @ 25°C



Companion Products
Materials Sold Separately
Notes
  • These fragments are best separated by Pulsed Field Gel Electrophoresis. Alternatively Lambda Mono Cut Mix can be separated by conventional gel electrophoresis using the following conditions: 0.5 μg Lambda Mono Cut Mix, 0.4 % agarose gel, 1X TAE, 20-30 V, 25°C for 24 hours.
  • 1X Gel Loading Dye, Purple, no SDS: 
    2.5% Ficoll®-400
    10mM EDTA
    3.3mM Tris-HCl (pH 8.0@25°C)
    0.02% Dye 1
    0.001% Dye 2
References
  • Daniels, D.L. et al. (1983). Appendix II:Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R.A. Weisberg(Ed.), Lambda-II. 519-676. New York: Cold Spring Harbor Laboratory Press.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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