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Catalog #	Concentration	Size	List Price	Quantity	Your Price
N0342S	50 µg/ml	50 gel lanes	$257.00		$231.30

Catalog #	Size	List Price	Your Price
N0342S	50 gel lanes	$257.00	$231.30

MidRange PFG Marker consists of concatemers of λ DNA isolated from the bacteriophage λ (cI857 ind1 Sam7) mixed with XhoI digested λ DNA embedded in 1% LMP agarose and supplied in a GelSyringe™ dispenser. XhoI produces fragments of 15.0 and 33.5 kb. These fragments anneal to and form concatemers with intact λ DNA. It is designed for use as a size marker for pulsed field gel electrophoresis (PFG). Size range: 15-291 kb.

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

N0342S -20

	MidRange PFG Marker (GelSyringe Dispenser)	N0342SVIAL	-20	1 x 50 gel lanes	50 µg/ml

Properties & Usage

Bases

Fragment	bp
1	15000
2	33500
3	48500
4	63500
5	82000
6	97000
7	112000
8	130500
9	145500
10	160500
11	179000
12	194000
13	209000
14	227500
15	242500
16	257500
17	276000
18	291000

Effective Size Range

15,000bp to 291,000bp

Storage Buffer

10 mM Tris-HCl
1 mM EDTA
50% Glycerol
1% LMP agarose
pH 8 @ 25°C

Companion Products

Notes

The photograph represents the pulsed field gel separation of Mid Range PFG Marker using a CHEF apparatus. The 1% agarose gel was run at 6 volts/cm using ramped pulse times from 1 to 25 seconds for 24 hours at 15°C in 0.5X TBE (50 mM Tris-HCl, 50 mM boric acid, 1 mM EDTA) made with Milli-Q™ water.
Extrude agarose from GelSyringe carefully and slice plugs from the end with a sharp blade. One plug is sufficient for one lane of a gel. Place the plug at the front of the well and seal with molten agarose just above gelling temperature (~42–45°C). Allow no bubbles to form.
Recommended plug sizes are from 5-10 µl. A 10 µl plug (one small graduation on the GelSyringe volume scale) contains approximately 0.5µg of DNA. Each GelSyringe yields 50+ plugs.
Melting plugs will cause denaturation of concatemers.
Never attach the agarose plugs to the gel comb before the gel is poured. Heat from the solidifying gel will cause the Lambda concatemers to denature.

References

Ellard, J., Greci, J. and Davis, T.B. unpublished observation.

FAQs

Why am I seeing poor results when running the PFG marker on my pulse field gel?

Citations

See more details on Bioz

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

MidRange PFG Marker

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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