Lambda DNA HindIII Digest

Catalog #	Concentration	Size	List Price	Quantity	Your Price
N3012L	500 µg/ml	750 gel lanes	$442.00		$397.80
N3012S	500 µg/ml	150 gel lanes	$111.00		$99.90

Catalog #	Size	List Price	Your Price
N3012L	750 gel lanes	$442.00	$397.80
N3012S	150 gel lanes	$111.00	$99.90

The HindIII digest of lambda DNA (cI857ind1 Sam 7) yields 8 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1). The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for approximating the mass of DNA in comparably intense samples of similar size.

Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS

Recommended gel percentage range: 0.6-1.2%
Optimum separation on 0.8%

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

N3012S -20

	Lambda DNA HindIII Digest	N3012SVIAL	-20	1 x 0.3 ml	500 µg/ml
	Gel Loading Dye, Purple (6X), no SDS	B7025SVIAL	25	1 x 1 ml	6 X

N3012L -20

	Lambda DNA HindIII Digest	N3012LVIAL	-20	2 x 0.75 ml	500 µg/ml
	Gel Loading Dye, Purple (6X), no SDS	B7025SVIAL	25	1 x 1 ml	6 X

Properties & Usage

Bases

Fragment	Mass (ng)	bp
1	477	23,130
2	194	9,416
3	135	6,557
4	90	4,361
5	48	2,322
6	42	2,027
7	12	564
8	3	125

Effective Size Range

125bp to 23,130bp

Storage Buffer

10 mM Tris-HCl
1 mM EDTA
pH 8 @ 25°C

Companion Products

Materials Sold Separately

Gel Loading Dye, Purple (6X), no SDS

Notes

For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH₂O and subsequently heated. Temperatures >60°C may cause denaturation.
The cohesive ends of fragments 1 and 4 may be separated by heating to 60°C for 3 minutes.
1X Gel Loading Dye, Purple, no SDS:
2.5% Ficoll®-400
10mM EDTA
3.3mM Tris-HCl (pH 8.0@25°C)
0.02% Dye 1
0.001% Dye 2

References

Daniels, D.L. et al. (1983). Appendix II:Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R.A. Weisberg(Ed.), Lambda-II. 519-676. New York: Cold Spring Harbor Laboratory Press.
Forster, A.C. et al. (1985). Nucl. Acids Res. 13, 745-761.

Protocols

Suggested Loading Protocol for N3014 & N3012

FAQs

Citations

See more details on Bioz

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

N3012S_L_v1

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

Legal And Disclaimer

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.