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Lambda DNA HindIII Digest

Catalog # Concentration Size List Price Quantity Your Price
N3012L 500 µg/ml 750 gel lanes $442.00
$397.80
N3012S 500 µg/ml 150 gel lanes $111.00
$99.90
Catalog # Size List Price Your Price
N3012L 750 gel lanes $442.00
$397.80
N3012S 150 gel lanes $111.00
$99.90
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • Size range: 125 bp to 23,130 bp
  • Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
  • Small size suitable for 150 gel lanes; large size suitable for 750 gel lanes

 

The HindIII digest of lambda DNA (cI857ind1 Sam 7) yields 8 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1). The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for approximating the mass of DNA in comparably intense samples of similar size.

Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS

  • Recommended gel percentage range: 0.6-1.2%
  • Optimum separation on 0.8% 
 
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Lambda DNA HindIII Digest N3012SVIAL -20 1 x 0.3 ml 500 µg/ml
  Gel Loading Dye, Purple (6X), no SDS B7025SVIAL 25 1 x 1 ml 6 X
  Lambda DNA HindIII Digest N3012LVIAL -20 2 x 0.75 ml 500 µg/ml
  Gel Loading Dye, Purple (6X), no SDS B7025SVIAL 25 1 x 1 ml 6 X

Properties & Usage

Bases

Fragment Mass (ng) bp
1 477 23,130
2 194 9,416
3 135 6,557
4 90 4,361
5 48 2,322
6 42 2,027
7 12 564
8 3 125

Effective Size Range

125bp to 23,130bp

Storage Buffer

10 mM Tris-HCl
1 mM EDTA
pH 8 @ 25°C



Companion Products
Materials Sold Separately
Notes
  • For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated. Temperatures >60°C may cause denaturation.
  • The cohesive ends of fragments 1 and 4 may be separated by heating to 60°C for 3 minutes.
  • 1X Gel Loading Dye, Purple, no SDS: 
    2.5% Ficoll®-400
    10mM EDTA
    3.3mM Tris-HCl (pH 8.0@25°C)
    0.02% Dye 1
    0.001% Dye 2
References
  • Daniels, D.L. et al. (1983). Appendix II:Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R.A. Weisberg(Ed.), Lambda-II. 519-676. New York: Cold Spring Harbor Laboratory Press.
  • Forster, A.C. et al. (1985). Nucl. Acids Res. 13, 745-761.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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