New England Biolabs Canada
Expand menu
Sign In or Sign UpMy NEB
1

PaqCI®

Catalog # Concentration Size List Price Quantity Your Price
R0745L 10000 units/ml 1000 units $670.00
$603.00
R0745S 10000 units/ml 200 units $161.00
$144.90
Catalog # Size List Price Your Price
R0745L 1000 units $670.00
$603.00
R0745S 200 units $161.00
$144.90
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
Featured Videos
View Video Library
  • Monarch_MiniprepProtocol_VideoThumb
    Golden Gate Assembly Workflow
  • Monarch_MiniprepProtocol_VideoThumb
    Golden Gate Assembly Domestication Tutorial
  • Monarch_MiniprepProtocol_VideoThumb
    Listen to DAD Informatics tools and NEB enzymes to enable complex one-pot Golden Gate Assemblies
  • Monarch_MiniprepProtocol_VideoThumb
    Listen to DAD (Data-optimized Assembly Design) when constructing high-complexity Golden Gate Assembly targets
  • Monarch_MiniprepProtocol_VideoThumb
    Golden Gate Assembly Tool Tutorial
  • Monarch_MiniprepProtocol_VideoThumb
    Function and Application of Type IIS Restriction Enzymes
PaqCI digests to completion and does not exhibit star activity



1 µg Lambda DNA was digested with 8 units of either PaqCI (NEB #R0745) or AarI (Thermo Fisher) following manufacturer’s recommended protocols. Digestions were analyzed on a 1% agarose gel. The results indicate that unlike AarI, PaqCI cuts to completion and does not exhibit star activity.
Product Source
An E. coli strain that carries the modified cloned PaqCI gene from Paucibacter aquatile.    
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  PaqCI® R0745SVIAL -20 1 x 0.02 ml 10,000 units/ml
  rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X
  PaqCI Activator S0532SVIAL -20 1 x 0.02 ml 20 µM
  PaqCI® R0745LVIAL -20 1 x 0.1 ml 10,000 units/ml
  rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X
  PaqCI Activator S0532LVIAL -20 1 x 0.1 ml 20 µM
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Companion Products
Materials Sold Separately
Notes
  • PaqCI is an isoschizomer of AarI.
  • This enzyme is impaired by CpG methylation. More information an be found at "Dam-Dcm and CpG Methylation."
  • PaqCI is an enzyme that requires multiple recognition sites to achieve complete digestion.  NEB has therefore included “PaqCI Activator” which can be added to the reaction with 1:1 ratio (i.e. 1 ul enzyme to 1 ul PaqCI Activator).  Please note that for Golden Gate Assembly reactions, the amount of PaqCI Activator varies depending on how many fragments are being assembled. 
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Change Notifications
PaqCI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10211228. All subsequent (higher number) lots will contain rAlbumin. For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin).
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Other Products You May Be Interested In
 Top