Esp3I

Catalog #	Concentration	Size	List Price	Quantity	Your Price
R0734L	10000 units/ml	1500 units	$568.00		$511.20
R0734S	10000 units/ml	300 units	$122.00		$109.80

Catalog #	Size	List Price	Your Price
R0734L	1500 units	$568.00	$511.20
R0734S	300 units	$122.00	$109.80

Product Source

An E. coli strain that carries the cloned Esp3I gene from Erwinia species RFL3 (A. Janulaitis).

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

R0734S -20

Esp3I	R0734SVIAL	-20	1 x 0.03 ml	10,000 units/ml
rCutSmart™ Buffer	B6004SVIAL	-20	1 x 1.25 ml	10 X
Gel Loading Dye, Purple (6X)	B7024AVIAL	25	1 x 0.5 ml	6 X

R0734L -20

Esp3I	R0734LVIAL	-20	1 x 0.15 ml	10,000 units/ml
rCutSmart™ Buffer	B6004SVIAL	-20	1 x 1.25 ml	10 X
Gel Loading Dye, Purple (6X)	B7024AVIAL	25	1 x 0.5 ml	6 X

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.

Reaction Conditions

1X rCutSmart™ Buffer
Incubate at 37°C

1X rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 Âµg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Activity in NEBuffers

NEBufferâ„¢ r1.1: 100%
NEBufferâ„¢ r2.1: 100%
NEBufferâ„¢ r3.1: <10%>
rCutSmartâ„¢ Buffer: 100%

Diluent Compatibility

Diluent B

Storage Buffer

10 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
500 Âµg/ml BSA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

65°C for 20 minutes

Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked

Companion Products

Materials Sold Separately

Notes

Esp3I is an isoschizomer of BsmBI-v2
This enzyme is blocked by Cpg methylation. More information can be found at "Dam-Dcm and CpG Methylation".

Protocols

Usage Guidelines

Selection Charts

Web Tools

FAQs

Troubleshooting

Restriction Enzyme Troubleshooting Guide

Citations

See more details on Bioz

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

R0734S_L_v1

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.