Lambda PFG Ladder

Product Information

Lambda PFG Ladder consists of one GelSyringe™ dispenser, sufficient for 50 gel lanes. Successively larger concatemers of lambda DNA (cI857 ind 1 Sam7) are embedded in 1% LMP agarose. This product is designed to be used as size markers for pulsed-field gel electrophoresis (PFG). Size range: 48.5–1,018 kb.

This product is related to the following categories:

DNA Markers & Ladders Products

This product can be used in the following applications:

DNA Analysis

• Reagents Supplied

  The following reagents are supplied with this product:

  NEB https://www.neb.com/en/#	Component Name	Component https://www.neb.com/en/#	Stored at (°C)	Amount	Concentration
  N0341S     -20       Lambda PFG Ladder (GelSyringe Dispenser) N0341SVIAL -20 1 x 50 gel lanes 50 µg/ml

• Properties & Usage

  Bases

  Fragment	bp
  1	1018500
  2	970000
  3	921500
  4	873000
  5	824500
  6	776000
  7	727500
  8	679000
  9	630500
  10	582000
  11	533500
  12	485000
  13	436500
  14	388000
  15	339500
  16	291000
  17	242500
  18	194000
  19	145500
  20	97000
  21	48500

  Effective Size Range

  48,500bp to 1,018,500bp

  Storage Buffer

  10 mM Tris-HCl
  1 mM EDTA
  50% Glycerol
  1% LMP agarose
  pH 8 @ 25°C

• Related Products

  Companion Products

  • λ DNA-Mono Cut Mix
  • MidRange PFG Marker

• Product Notes

  1. The photograph represents the pulsed field gel separation of Lambda Ladders using a CHEF apparatus. The 1% agarose gel was run at 4.5 volts/cm using ramped pulse times from 5 to 120 seconds for 48 hours at 15°C in 0.5X TBE (50 mM Tris-HCl, 50 mM boric acid, 1 mM EDTA) made with Milli-Q™ water allowing resolution of 15 to 21 Lambda Ladder bands.
  2. Recommended plug sizes are from 5-10 µl. A 10 µl plug (one small graduation on the GelSyringe volume scale) contains approximately 0.5 µg of DNA. Each GelSyringe yields 50+ plugs.
  3. Extrude agarose from GelSyringe carefully and slice plugs from the end with a sharp blade. One plug is sufficient for one lane of a gel. Place the plug at the front of the well and seal with molten agarose just above gelling temperature (~42–45°C). Allow no bubbles to form.
  4. Melting plugs will cause denaturation of concatemers.
  5. Never attach the agarose plugs to the gel comb before the gel is poured. Heat from the solidifying gel will cause the Lambda concatemers to denature.

• References

  1. Burke-Aguaro, D. unpublished observation.
  2. Ellard, J., Greci, J. and Davis, T.B. unpublished observation.

FAQs & Troubleshooting

• FAQs

  1. Why am I seeing poor results when running the PFG marker on my pulse field gel?

Citations & Technical Literature

• Citations

  Product Citation Tool

Quality, Safety & Legal

• Quality Control Assays

  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

• Certificate of Analysis

  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

  • N0341S_v1_0021606
  • N0341S_v1_0041706
  • N0341S_v1_10017974
  • N0341S_v1_10040190
  • N0341S_v1_10058457
  • N0341S_v1_10081430
  • N0341S_v1_10107782
  • N0341S_v1_10141644
  • N0341S_v1_10175318
  • N0341S_v1_10180292
  • N0341S_v1_10197915
  • N0341S_v1_10206251
  • N0341S_v1_10216924
  • N0341S_v1_10221508
  • N0341S_v1_10247519
  • N0341S_v1_10269470
  • N0341S_v1_10275476

• Safety Data Sheets

  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

  • Lambda PFG Ladder

• Legal and Disclaimers

  Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.