Quick CIP

Catalog #	Concentration	Size	List Price	Quantity	Your Price
M0525L	5000 units/ml	5000 units	$693.00		$623.70
M0525S	5000 units/ml	1000 units	$145.00		$130.50

Catalog #	Size	List Price	Your Price
M0525L	5000 units	$693.00	$623.70
M0525S	1000 units	$145.00	$130.50

Quick CIP is a heat-labile recombinant version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source. Quick CIP nonspecifically catalyzes the dephosphorylation of 5′ and 3′ ends of DNA and RNA phosphomonoesters. It also hydrolyses ribo- and deoxyribonucleoside triphosphates (NTPs and dNTPs). Quick CIP is useful in many applications that require the dephosphorylation of DNA or RNA ends. In cloning, dephosphorylation prevents re-ligation of linearized plasmid DNA. The enzyme can quickly dephosphorylate 5′ protruding, 5′ recessed, and blunt ends in just 10 minutes. Quick CIP may also be used to degrade unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis.

Quick CIP is completely and irreversibly inactivated by heating it at 80°C for 2 minutes, unlike wild type CIP, which is not heat-inactivatable. This makes removal of Quick CIP prior to ligation or end-labeling unnecessary.

Product Source

A Pichia pastoris clone that carries the calf intestinal alkaline phosphatase gene from calf intestinal mucosa.

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

M0525S -20

	Quick CIP	M0525SVIAL	-20	1 x 0.2 ml	5,000 units/ml
	rCutSmart™ Buffer	B6004SVIAL	-20	1 x 1.25 ml	10 X

M0525L -20

	Quick CIP	M0525LVIAL	-20	1 x 1 ml	5,000 units/ml
	rCutSmart™ Buffer	B6004SVIAL	-20	1 x 1.25 ml	10 X

Features

Rapid and irreversible heat inactivation eliminates unwanted activity
Improved storage stability versus native enzyme
Faster reaction setup (no supplemental additives like zinc required) and shorter incubation time
Flexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)
Less enzyme required (high specific activity), resulting in a lower cost per reaction
No need for multiple phosphatases (Quick CIP removes 5′- and 3′- phosphates from DNA, RNA and dNTPs )
Active on unincorporated dNTPs in PCR products - improves DNA sequencing and SNP analysis
Recombinant for purity, consistency and value

Application Features

Dephosphorylation 5′ and 3′ ends of DNA and RNA.
Dephosphorylation of cloning vector DNA to prevent recircularization during ligation.
Dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase.
Removal of dNTPs in PCR reactions prior to sequencing or SNP analysis.

An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Companion Products

Notes

Quick CIP, as are most alkaline phosphatases, is a Zn₂+ and Mg₂+-dependent enzyme. Our formulation has tightly bound zinc atoms in the active center and does not require supplemental zinc or other additives.
Quick CIP is also active in 1X NEBuffers 1.1, 2.1, 3.1 as well as NEBuffers 1, 2, 3 and 4 and NEBuffer for EcoRI.
Quick CIP activity is enhanced in the presence of monovalent salts.
Quick CIP is inhibited by metal chelators (e.g. EDTA), inorganic phosphate and phosphate analogs.
The Quick CIP activity is decreased in the presence of reducing agents (DTT, β-mercaptoethanol).
Quick CIP is a homodimer. The molecular weight of the monomer is 56 kDa.

References

Weissig, H. et al (1993). Biochem. J. 290, 503-508.
Manes, T (1998). J. Biol. Chem. 273, 23353-23360.

Protocols

FAQs

Citations

See more details on Bioz

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

M0525S_L_v1

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.