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Exonuclease I (E. coli)

Catalog # Concentration Size List Price Quantity Your Price
M0293L 20000 units/ml 15000 units $461.00
$414.90
M0293S 20000 units/ml 3000 units $115.00
$103.50
Catalog # Size List Price Your Price
M0293L 15000 units $461.00
$414.90
M0293S 3000 units $115.00
$103.50
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

  • DNA specific exonuclease
  • Catalyzes the removal of nucleotides from linear single-stranded DNA in the 3' to 5' direction
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

Exonuclease I is ideal for:

  • Removal of single-stranded primers in PCR reactions prior to Sanger DNA sequencing or SNP analysis
  • Removal of single-stranded primers for nested PCR reactions
  • Removal of linear single-stranded DNA, leaving behind double-stranded DNA in the sample

Note: See also Exo-CIP™ Rapid PCR Cleanup Kit (NEB #E1050).

Featured Videos
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  • Monarch_MiniprepProtocol_VideoThumb
    What are exonucleases and their applications?
Highlights
  • Isolated from a recombinant source
  • 3' → 5' single strand exonuclease
  • Supplied with 10X Reaction Buffer
Product Source
An E. coli strain that carries the cloned Exo I gene from E. coli NM554.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Exonuclease I (E. coli) M0293SVIAL -20 1 x 0.15 ml 20,000 units/ml
  Exonuclease I Reaction Buffer B0293SVIAL -20 1 x 1 ml 10 X
  Exonuclease I (E. coli) M0293LVIAL -20 1 x 0.75 ml 20,000 units/ml
  Exonuclease I Reaction Buffer B0293SVIAL -20 1 x 1 ml 10 X
Application Features
  • Exonuclease I degrades excess single-stranded primer oligonucleotide from a reaction mixture containing double-stranded extension products

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the release of 10 nmol of acid-soluble nucleotide in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X Exonuclease I Reaction Buffer with 0.17 mg/ml single-stranded [3H]-DNA.

Reaction Conditions

1X Exonuclease I Reaction Buffer
Incubate at 37°C

1X Exonuclease I Reaction Buffer
67 mM Glycine-KOH
6.7 mM MgCl2
10 mM β-ME
(pH 9.5 @ 25°C)

Usage Concentration

10,000 units/ml

Storage Buffer

10 mM Tris-HCl
100 mM NaCl
5 mM β-ME
0.5 mM EDTA
100 µg/ml BSA
50% Glycerol
pH 7.5 @ 25°C

Heat Inactivation

80°C for 20 minutes

Companion Products
Materials Sold Separately
References
  • Lehman and Nussbaum (1964). J. Biol. Chem. 239,
  • Kusher, et al. (1971). Proc. Natl. Acad. Sci. USA. 68,
  • Kusher et al. (1972). Proc. Natl. Acad. Sci. USA. 69, 1366.
  • Goldmark and Linn (1972). J. Biol. Chem. 247,
  • Rosamond et al. (1979). J. Biol. Chem. 254,
Citations
Additional Citations
  • Enroth, C.H., Fehler, A.O., Poulsen, L.D., Vinther, J. (2019) Excess primer degradation by Exo I improves the preparation of 3´ cDNA ligation-based sequencing libraries. Biotechniques 67, 110-116. DOI: 10.2144/btn-2018-0178
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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