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Thermolabile Exonuclease I

Catalog # Concentration Size List Price Quantity Your Price
M0568L 20000 units/ml 15000 units $503.00
$452.70
M0568S 20000 units/ml 3000 units $123.00
$110.70
Catalog # Size List Price Your Price
M0568L 15000 units $503.00
$452.70
M0568S 3000 units $123.00
$110.70
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

  • DNA specific exonuclease
  • Catalyzes the removal of nucleotides from linear single-stranded DNA in the 3' to 5' direction
  • Unlike Exonuclease I (NEB #M0293), Thermolabile Exonuclease I can be quickly heat inactivated at 80°C in 1 minute
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

Thermolabile Exonuclease I is ideal for:

  • Removal of single-stranded primers in PCR reactions prior to DNA sequencing or SNP analysis
  • Removal of single-stranded primers for nested PCR reactions
  • Removal of linear single-stranded DNA, leaving behind double-stranded DNA in the sample
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Thermolabile Exonuclease I catalyzes the removal of nucleotides from single-stranded DNA (ssDNA) in the 3′ to 5′ direction.

 

Figure 1. Heat Inactivation Profile of Thermolabile ExoI



Thermolabile ExoI can be heat inactivated quickly to speed up downstream applications. This data demonstrates that Thermolabile ExoI can be heat inactivated at 80°C for 1 min, 65°C for 4 min or 60°C for 15 min in both NEBuffer 3.1 as well as Standard Taq Reaction Buffer.
Product Source
An E. coli strain that carries a cloned Thermolabile Exonuclease I gene originating from a psychrophilic marine bacterium.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Thermolabile Exonuclease I M0568SVIAL -20 1 x 0.15 ml 20,000 units/ml
  NEBuffer™ r3.1 B6003SVIAL -20 1 x 1.25 ml 10 X
  Thermolabile Exonuclease I M0568LVIAL -20 1 x 0.75 ml 20,000 units/ml
  NEBuffer™ r3.1 B6003SVIAL -20 1 x 1.25 ml 10 X
Application Features
  • Removal of single-stranded primers in PCR reactions prior to DNA sequencing or SNP analysis
  • Removal of single-stranded primers for nested PCR reactions
  • Removal of single-stranded DNA from dsDNA
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Companion Products
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Notes
  • This enzyme can be heat inactivated at 80°C for 1 minute, 65°C for 5 minutes or 60°C for 15 minutes.
  • Thermolabile ExoI is active in the presence of 25 mM to 200 mM NaCl.
  • If the amount of ssDNA to be degraded needs to be increased, we recommend adding more enzyme instead of extending the reaction time.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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